Fractionating power and outlet stream polydispersity in asymmetrical flow field-flow fractionation. Part II: programmed operation

被引:5
作者
Williams, P. Stephen [1 ]
机构
[1] Cambrian Technol Inc, 1772 Saratoga Ave, Cleveland, OH 44109 USA
关键词
Asymmetrical flow field-flow fractionation; Programmed operation; Fractionating power; Outlet stream polydispersity; HYDRODYNAMIC RADIUS DETERMINATION; POLARIZATION CHROMATOGRAPHY; NONEQUILIBRIUM THEORY; SECONDARY RELAXATION; SIZE; SEPARATION; CHANNEL; PARTICLES; DIFFUSION; CELLULOSE;
D O I
10.1007/s00216-016-0007-x
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Asymmetrical flow field-flow fractionation (As-FlFFF) is a widely used technique for analyzing polydisperse nanoparticle and macromolecular samples. The programmed decay of cross flow rate is often employed. The interdependence of the cross flow rate through the membrane and the fluid flow along the channel length complicates the prediction of elution time and fractionating power. The theory for their calculation is presented. It is also confirmed for examples of exponential decay of cross flow rate with constant channel outlet flow rate that the residual sample polydispersity at the channel outlet is quite well approximated by the reciprocal of four times the fractionating power. Residual polydispersity is of importance when online MALS or DLS detection are used to extract quantitative information on particle size or molecular weight. The theory presented here provides a firm basis for the optimization of programmed flow conditions in As-FlFFF.
引用
收藏
页码:317 / 334
页数:18
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