Water-Soluble Fluorescent Probe with Dual Mitochondria/Lysosome Targetability for Selective Superoxide Detection in Live Cells and in Zebrafish Embryos

被引：48

作者：

Lu, Xiuhong ^{[1
,3
]}

Chen, Zhongjian ^{[4
]}

Dong, Xiaochun ^{[1
]}

Zhao, Weili ^{[1
,2
]}

机构：

[1] Fudan Univ, Sch Pharm, 826 Zhangheng Rd, Shanghai 201203, Peoples R China

[2] Henan Univ, Minist Educ, Key Lab Special Funct Mat, Kaifeng 475004, Peoples R China

[3] Fudan Univ, Huashan Hosp, PET Ctr, 518 East Wuzhong Rd, Shanghai 200235, Peoples R China

[4] Shanghai Dermatol Hosp, Shanghai 200443, Peoples R China

来源：

ACS SENSORS | 2018年 / 3卷 / 01期

关键词：

fluorescent probe; fluorescein; KO2; superoxide; mitochondria; -targeting; lysosome-targeting; dual targetable; IN-VIVO; OXIDATIVE STRESS; CANCER CELLS; ANION; MECHANISM; DESIGN; NEUTROPHILS; CHEMISTRY; RADICALS; BIOLOGY;

D O I：

10.1021/acssensors.7b00831

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel water-soluble fluorescein-based fluorescent probe for superoxide detection was developed. The probe is fairly stable under neutral and acidic conditions. It can be used to detect superoxide both in solution with the detection limit of 2.2 mu M and in living cells. Cell imaging experiments indicated that such a probe displayed good cell penetration and O-2(center dot-) could be detected with PMA-stimulated HepG2 cells in both mitochondria and lysosome. Such a probe is the first dual mitochondria and lysosome-targetable fluorescent chemodosimeter. Additionally, O-2(center dot-) in intact live zebrafish embryos was successfully visualized under PMA-stimulated conditions, and the possible detection mechanism was studied as well.

引用

页码：59 / 64

页数：6

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