Phototoxicity of Kava - Formation of Reactive Oxygen Species Leading to Lipid Peroxidation and DNA Damage

被引：0

作者：

Xia, Qingsu ^{[1
]}

Chiang, Hsiu-Mei ^{[2
]}

Zhou, Yu-Ting ^{[3
]}

Yin, Jun-Jie ^{[3
]}

Liu, Fang ^{[1
]}

Wang, Cheng ^{[1
]}

Guo, Lei ^{[1
]}

Fu, Peter P. ^{[1
]}

机构：

[1] US FDA, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA

[2] China Med Univ, Dept Cosmeceut, Taichung, Taiwan

[3] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA

来源：

AMERICAN JOURNAL OF CHINESE MEDICINE | 2012年 / 40卷 / 06期

关键词：

Kava Extract; Photosensitivity; UVA Light; Reactive Oxygen Species; Lipid Peroxidation; HaCaT Keratinocytes; 8-OHdG; SINGLET OXYGEN; FREE-RADICALS; UVA; INDUCTION; PHOTOIRRADIATION; FIBROBLASTS; SUPPLEMENTS; SUPEROXIDE; DERMOPATHY; CHEMISTRY;

D O I：

10.1142/S0192415X12500942

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Kava is one of the most widely sold herbal dietary supplements in the United States. It has been reported that, besides exhibiting hepatotoxicity, kava also possesses photosensitivity and induces dermopathy in humans. In this study, we determined that UVA irradiation of kava in the presence of a lipid, methyl linoleate, generated lipid peroxidation which was mediated by singlet oxygen generated during photoirradiation. The six major kavalactones (yangonin, 7,8-dihydrokawa in, kawain, 7,8-dihydromethysticin, methysticin, and 5,6-dehydrokawain) were also studied in parallel; only 5,6-dehydrokawain and yangonin-induced a low level of lipid peroxidation. UVA irradiation of kava in human HaCaT skin keratinocytes induced cytotoxicity which was mediated by oxidative stress, led to DNA strand cleavage, and produced 8-hydroxy-2'-deoxyguanosine (8-OHdG) adduct. Study by the electron spin resonance (ESR) method revealed that UVA irradiation of kava produced singlet oxygen and carbon-centered radicals. The overall results suggest that kava is photocytotoxic and photo-genotoxic, both mediated by free radicals generated during photoirradiation.

引用

页码：1271 / 1288

页数：18

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