Rapid Genotyping of Human Papillomavirus by Desorption Electrospray Ionization Mass Spectrometry

被引：1
作者：
Kim, Shin Hye [1 ,2 ]
Hong, Sun Pyo [3 ]
Cho, Woojae [3 ]
Kim, Jeongkwon [2 ]
Lee, Tae Geol [1 ]
Yoon, Sohee [1 ]
机构：
[1] Korea Res Inst Stand & Sci KRISS, Ctr Nanobio Measurement, Daejeon 34113, South Korea
[2] Chungnam Natl Univ, Dept Chem, Daejeon 34134, South Korea
[3] GeneMatrix Inc, R&D Ctr, Seongnam 13543, South Korea
来源：
BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2017年 / 38卷 / 06期
关键词：
Desorption electrospray ionization; Mass spectrometry; Human papillomavirus; Restriction fragment mass polymorphism; SAMPLE PREPARATION;
D O I：
10.1002/bkcs.11130
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
This study describes a new approach to rapidly detecting of human papillomavirus (HPV) DNA using desorption electrospray ionization mass spectrometry (DESI-MS) without sample preparation such as extraction or purification. Our strategy utilizes the ambient ionization mass spectrometry for ionizing and detecting oligonucleotides from restriction fragment mass polymorphism (RFMP) assay, and is to identify which HPV genotype from combinations of multiply charged ions of oligonucleotides. In DESI-MS, the spray solvent system operates at the stage of washing a complex mixture of polymerase chain reaction (PCR) products, so that the oligonucleotides can be detected without sample treatment. The study was verified to rapid diagnosis of cervical cancer by detecting and genotyping of HPV DNA based RFMP assay using DESI-MS.
引用
收藏
页码：603 / 606
页数：4
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