Impact of different nitrogen sources on the growth of Arthrospira sp PCC 8005 under batch and continuous cultivation - A biochemical, transcriptomic and proteomic profile

被引：32

作者：

Deschoenmaeker, Frederic ^{[1
]}

Bayon-Vicente, Guillaume ^{[1
]}

Sachdeva, Neha ^{[1
]}

Depraetere, Orily ^{[2
]}

Pino, Juan Carlos Cabrera ^{[3
]}

Leroy, Baptiste ^{[1
]}

Muylaert, Koenraad ^{[2
]}

Wattiez, Ruddy ^{[1
]}

机构：

[1] Univ Mons, Res Inst Biosci, Dept Prote & Microbiol, Pl Parc 20, B-7000 Mons, Belgium

[2] Katholieke Univ Leuven, Lab Aquat Biol, Campus Kortrijk,E Sabbelaan 53, B-8500 Kortrijk, Belgium

[3] Mat Nova, Biotechnol Unit, Rue Foudriers 1, B-7822 Ghislenghien, Belgium

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 237卷

关键词：

Arthrospira sp PCC 8005; Nitrogen metabolism; Omics; Photobioreactor; Waste recycling; PHOTOSYSTEM-II; AMMONIUM ASSIMILATION; NITRATE ASSIMILATION; PLATENSIS; BIOMASS; TEMPERATURE; CHLORELLA; TOXICITY; PHOTOSYNTHESIS; INHIBITION;

D O I：

10.1016/j.biortech.2017.03.145

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The aim of the present study was to evaluate the effects of varying concentrations of different nitrogen sources (individually or in combination) on the biochemical, transcriptomic and proteomic profiles of Arthrospira sp. PCC 8005 under batch and continuous modes. In batch mode, while ammonium showed a repressive effect on nitrate-assimilation pathway of the cyanobacteria; better growth and nutrient uptake rate were observed in presence of urea than nitrate. The inhibitory effect of ammonium was further confirmed by the continuous photobioreactor study wherein the nutrient feed was transiently replaced from nitrate to ammonium (28 mM turbiostat regime). The changes in lipid, exopolysaccharide, transcriptomic and proteomic profiles of cyanobacteria on transition from nitrate to ammonium indicated at an onset of nutrient stress. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：78 / 88

页数：11

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