Glyceraldehyde-3-phosphate dehydrogenase of Mycoplasma pneumoniae induces infection-related glomerulonephritis

被引：14

作者：

Hirano, Daishi ^{[1
]}

Oda, Takashi ^{[2
]}

Ito, Akira ^{[1
]}

Yamada, Akifumi ^{[1
]}

Kakegawa, Daisuke ^{[1
,3
]}

Miwa, Saori ^{[1
]}

Umeda, Chisato ^{[1
]}

Takemasa, Yoichi ^{[1
]}

Tokunaga, Ai ^{[1
]}

Wajima, Takeaki ^{[4
]}

Nakaminami, Hidemasa ^{[4
]}

Noguchi, Norihisa ^{[4
]}

Ida, Hiroyuki ^{[1
]}

机构：

[1] Jikei Univ, Dept Pediat, Sch Med, Tokyo, Japan

[2] Tokyo Med Univ, Kidney Dis Ctr, Dept Nephrol & Blood Purificat, Hachioji Med Ctr, Tokyo, Japan

[3] Saitama Childrens Med Ctr, Div Nephrol, Saitama, Japan

[4] Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Microbiol, Tokyo, Japan

来源：

CLINICAL NEPHROLOGY | 2019年 / 92卷 / 05期

关键词：

Mycoplasma pneumoniae; glyceraldehyde-3-phosphate dehydrogenase; nephritis-associated plasmin receptor; acute poststreptococcal glomerulonephritis; PLASMIN RECEPTOR; POSTSTREPTOCOCCAL GLOMERULONEPHRITIS; NEPHRITIS; ANTIGEN;

D O I：

10.5414/CN109786

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Infection-related glomerulonephritis (IRGN) was previously thought to be due mostly to Streptococcus species, but is now known to be caused by a variety of other pathogens. Nephritis-associated plasmin receptor (NAPlr) was originally isolated from group A streptococci as the protein responsible for acute poststreptococcal glomerulonephritis, and was shown to be identical to streptococcal glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Here, we describe a 7-year-old boy diagnosed with Mycoplasma pneumoniae IRGN presenting with acute nephritic syndrome. Laboratory data revealed a significant increase in serum anti-M. pneumoniae antibody titer. Renal biopsy revealed diffuse global endocapillary proliferation and cellular crescents in 5/43 glomeruli examined. Although antistreptolysin O antibody titer and serum complement C3 level were within the respective normal ranges, glomeruli showed positive staining for NAPlr and upregulation of plasmin activity. In addition, positive staining for NAPlr in the glomeruli was abolished by preabsorption of anti-NAPlr antibody with recombinant M. pneumoniae GAPDH. Western blotting analysis revealed anti-NAPlr antibody reactivity with a band at around the predicted size of GAPDH in the protein isolate of M. pneumoniae (37 kDa). Furthermore, immobilized M. pneumoniae GAPDH bound to anti-NAPlr antibody as well as plasmin in vitro. These data suggest that M. pneumoniae GAPDH has a function similar to streptococcal GAPDH (NAPlr) and may induce plasmin-related glomerular damage in M. pneumoniae IRGN. NAPlr could be a marker of glomerulonephritis related to infection not only by streptococci but also by M. pneumoniae.

引用

页码：263 / 272

页数：10

共 20 条

[1] Glyceraldehyde-3-phosphate dehydrogenase of Streptococcus pneumoniae is a surface-displayed plasminogen-binding protein [J].