Uneven modulation of the annexin 1 system in osteoblast-like cells by dexamethasone

被引：4
作者：
Giner, Rosa M.
Mancini, Lucia
Kamal, Ahmad M.
Perretti, Mauro [1 ]
机构：
[1] William Harvey Res Inst, London, England
[2] Univ Valencia, Dept Farmacol, Valencia, Spain
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 354卷 / 02期
基金：
英国惠康基金;
关键词：
FPRL-1; Saos-2; annexin A1; dexamethasone; FPR; interleukin-6; mRNA;
D O I：
10.1016/j.bbrc.2006.12.224
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We tested whether glucocorticoids modulated osteoblast expression of the annexin 1 system, including the ligand and two G-coupled receptors termed formyl-peptide receptor (FPR) and FPR-like-1 (FPRL-1). In Saos-2 cells, rapid up-regulation of FPR mRNA upon cell incubation with dexamethasone (0.01-1 mu M) was observed, with significant changes as early as 2 h and a more marked response at 24 h; annexin 1 and FPRL-1 mRNA changes were more subtle. At the protein level, dexamethasone provoked a rapid externalization of annexin 1 (maximal at 2 h) followed by delayed time-dependent changes in the cell cytosol. Saos-2 cell surface expression of FPR or FPRL-1 could not be detected, even when dexamethasone was added with the bone modelling cytokines interleukin-6 or interleukin-1. The uneven modulation of the annexin 1 system (mediator and its putative receptors) in osteoblasts might lead to a better understanding of how these complex biochemical pathways become operative in bone. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：414 / 419
页数：6
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