RAPD differentiation of Malassezia spp. from cattle, dogs and humans

被引：13

作者：

Duarte, Eduardo R. ^{[1
]}

Hamdan, Junia S. ^{[2
]}

机构：

[1] Univ Fed Minas Gerais, Inst Ciencias Agr, BR-39400006 Montes Claros, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, Belo Horizonte, MG, Brazil

来源：

MYCOSES | 2010年 / 53卷 / 01期

关键词：

Domestic animals; epidemiology; Malassezia spp; random amplified polymorphic DNA-polymerase chain reaction; INTENSIVE-CARE UNIT; PITYRIASIS-VERSICOLOR; SEBORRHEIC DERMATITIS; ATOPIC-DERMATITIS; DOMESTIC-ANIMALS; HEALTHY-SUBJECTS; MINAS-GERAIS; PACHYDERMATIS; FURFUR; IDENTIFICATION;

D O I：

10.1111/j.1439-0507.2008.01658.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

P>The molecular characterization of Malassezia spp. isolates from animals and humans has not been thoroughly studied. We have analysed the DNA profile by random amplified polymorphic DNA (RAPD)-PCR to compare the genetic diversity between isolates from the external ears of cattle, dogs and humans. The analysis of electrophoretic profiles on 8% polyacrylamide gel and their phenograms showed genetic heterogeneity between RAPD profiles of Malassezia furfur and Malassezia slooffiae isolates from humans and cattle and between Malassezia pachydermatis isolates from dogs and cattle. Intra-species variations in DNA pattern of Malassezia isolates and the presence of specific genetic types in cattle, dogs or humans were observed. A review of genetic heterogeneity of these yeast in veterinary and human medicine studies is given considering a possible transmission animal to human or human to animal. Additional studies must clarify the differences between the RAPD band patterns observed in this and other studies, which would facilitate monitoring of Malassezia spp. carriage in domestic animals and in humans.

引用

页码：48 / 56

页数：9

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