Cell-surface display of the active mannanase in Yarrowia lipolytica with a novel surface-display system

被引：18

作者：

Yang, Xiao-Song ^{[1
]}

Jiang, Zheng-Bing ^{[1
]}

Song, Hui-Ting ^{[2
]}

Jiang, Si-Jing ^{[1
]}

Madzak, Catherine ^{[3
]}

Ma, Li-Xin ^{[1
]}

机构：

[1] Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China

[2] Hubei Univ, Coll Resources & Environm Sci, Wuhan 430062, Peoples R China

[3] INRA CNRS AgroParisTech CBAI, UMR Microbiol & Genet Mol, F-78850 Thiverval Grignon, France

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2009年 / 54卷

基金：

中国国家自然科学基金;

关键词：

cell-surface display; Flolp; mannanase; whole-cell biocatalyst; Yarrowia lipolytica; SACCHAROMYCES-CEREVISIAE; PROTEIN EXPRESSION; YEAST; CONSTRUCTION; CLONING; SECRETION; GROWTH;

D O I：

10.1042/BA20090222

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel surface-display system was constructed using the cell-wall anchor protein Flolp from Saccharomyces cerevisiae, the mannanase (man I) from Bacillus subtilis fused with the C-terminus of Flolp and the 6 x His tag was inserted between Flolp and manl. The fusion protein was displayed on the cell surface of Yarrowia lipolytica successfully, and it was confirmed by immunofluorescence. In succession, the surface-displayed mannanase was characterized. The optimum catalytic conditions for the recombinant mannanase were 55 degrees C at pH 6.0, and it exhibited high stability against pH variation. The highest activity of the recombinant mannanase reached 62.3 IU/g (dry cell weight) after the recombinant was cultivated for 96 h in YPD medium [1% (w/v) yeast extract/2% (w/v) peptone/2% (w/v) glucose]. To our knowledge, the present paper is the first to report that high-activity mannanase is displayed on the cell surface of Y. lipolytica with Flolp.

引用

页码：171 / 176

页数：6

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