The Development of Genipin-Crosslinked Poly(caprolactone) (PCL)/Gelatin Nanofibers for Tissue Engineering Applications

被引：149

作者：

Kim, Min Sup ^{[2
]}

Jun, Indong ^{[1
]}

Shin, Young Min ^{[1
]}

Jang, Wonhee ^{[3
]}

Kim, Sun I. ^{[2
]}

Shin, Heungsoo ^{[1
]}

机构：

[1] Hanyang Univ, Dept Bioengn, Seoul 133791, South Korea

[2] Hanyang Univ, Dept Biomed Engn, Seoul 133791, South Korea

[3] Dongguk Univ, Dept Life Sci, Seoul 100715, South Korea

来源：

MACROMOLECULAR BIOSCIENCE | 2010年 / 10卷 / 01期

关键词：

electrospinning; gelatin; polycaprolactone; skeletal muscles; tissue engineering; HUMAN SKELETAL-MUSCLE; BIOLOGICAL TISSUE; LINKING REACTION; CELL THERAPY; IN-VITRO; SCAFFOLDS; GELATIN; DIFFERENTIATION; REGENERATION; MYOBLASTS;

D O I：

10.1002/mabi.200900168

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Composite nano-fibers of poly(caprolactone) (PCL) and gelatin crosslinked with genipin are prepared. The contact angles and mechanical properties of crosslinked PCL-gelatin nanofibers decrease as the gelatin content increases. The proliferation of myoblasts is higher in the crosslinked PCL-gelatin nanofibers than in the PCL nanofibers, and the formation of myotubes is only observed on the crosslinked PCL-gelatin nanofibers. The expression level of myogenin, myosin heavy chain, and troponin T genes is increased as the gelatin content is increased. The results suggest that PCL-gelatin nanofibers crosslinked with genipin can be used as a substrate to modulate proliferation and differentiation of myoblasts, presenting potential applications in muscle tissue engineering.

引用

页码：91 / 100

页数：10

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