Development of primer and probe sets for the detection of plant species in honey

被引:84
作者
Laube, I. [1 ]
Hird, H. [2 ]
Brodmann, P. [3 ]
Ullmann, S. [4 ]
Schoene-Michling, M. [3 ]
Chisholm, J. [2 ]
Broll, H. [1 ]
机构
[1] BfR Prod Ident Traceabil & Novel Food, D-14195 Berlin, Germany
[2] Cent Sci Lab, York YO41 1LZ, N Yorkshire, England
[3] Biolytix AG, CH-4108 Witterswil, Switzerland
[4] Qiagen, D-40724 Hilden, Germany
关键词
Plant species; Real-time PCR; Honey; Rape; Rockrose; Sweet chestnut; Olive; DNA;
D O I
10.1016/j.foodchem.2008.09.063
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
An objective of workpackage 3 within the European Research Project "TRACE" was the identification of PCR markers for the detection of plant species related to honey. Within the project "Miel de Corse", a protected designation of origin (PDO) honey and "Miel de Galicia", a protected geographical indication (PGI) honey as well as German and English honeys were analysed. Beside the development of plant species specific real-time PCR systems a method has been established for the detection of DNA derived from plant species in general. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:979 / 986
页数:8
相关论文
共 6 条
[1]  
BENSON DA, 2005, NUCLEIC ACIDS RES, V38, P33
[2]   DETECTION OF SPECIFIC POLYMERASE CHAIN-REACTION PRODUCT BY UTILIZING THE 5'-]3' EXONUCLEASE ACTIVITY OF THERMUS-AQUATICUS DNA-POLYMERASE [J].
HOLLAND, PM ;
ABRAMSON, RD ;
WATSON, R ;
GELFAND, DH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :7276-7280
[3]   METHODS OF MELISSOPALYNOLOGY [J].
LOUVEAUX, J ;
MAURIZIO, A ;
VORWOHL, G .
BEE WORLD, 1978, 59 (04) :139-157
[4]   UNIVERSAL PRIMERS FOR AMPLIFICATION OF 3 NONCODING REGIONS OF CHLOROPLAST DNA [J].
TABERLET, P ;
GIELLY, L ;
PAUTOU, G ;
BOUVET, J .
PLANT MOLECULAR BIOLOGY, 1991, 17 (05) :1105-1109
[5]   RANDOM AMPLIFIED POLYMORPHIC DNA AND PEDIGREE RELATIONSHIPS IN SPRING BARLEY [J].
TINKER, NA ;
FORTIN, MG ;
MATHER, DE .
THEORETICAL AND APPLIED GENETICS, 1993, 85 (08) :976-984
[6]  
Vaerman JL, 2004, J BIOL REG HOMEOS AG, V18, P212