Acute Oral Toxicity of Tetrodotoxin in Mice: Determination of Lethal Dose 50 (LD50) and No Observed Adverse Effect Level (NOAEL)

被引：51

作者：

Abal, Paula ^{[1
]}

Louzao, M. Carmen ^{[1
]}

Antelo, Alvaro ^{[2
]}

Alvarez, Mercedes ^{[2
]}

Cagide, Eva ^{[2
]}

Vilarino, Natalia ^{[1
]}

Vieytes, Mercedes R. ^{[3
]}

Botana, Luis M. ^{[1
]}

机构：

[1] Univ Santiago Compostela, Fac Vet, Dept Farmacol, Lugo 27002, Spain

[2] Laboratorio CIFGA SA, Plaza Santo Domingo 20-5, Lugo 27001, Spain

[3] Univ Santiago Compostela, Fac Vet, Dept Fisiol, Lugo 27002, Spain

来源：

TOXINS | 2017年 / 9卷 / 03期

关键词：

acute oral toxicity; European molluscs; lethal dose 50 (LD50); No Observed Adverse Effect Level (NOAEL); risk assessment; tetrodotoxin; NEUROTOXIN; ORIGIN; MS/MS;

D O I：

10.3390/toxins9030075

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Tetrodotoxin (TTX) is starting to appear in molluscs from the European waters and is a hazard to seafood consumers. This toxin blocks sodium channels resulting in neuromuscular paralysis and even death. As a part of the risk assessment process leading to a safe seafood level for TTX, oral toxicity data are required. In this study, a 4-level Up and Down Procedure was designed in order to determine for the first time the oral lethal dose 50 (LD50) and the No Observed Adverse Effect Level (NOAEL) in mice by using an accurate well-characterized TTX standard.

引用

页数：6

共 24 条

[1] Tetrodotoxin: Chemistry, Toxicity, Source, Distribution and Detection [J].