A Competitive Pull-Down Assay Using G-quadruplex DNA Linked Magnetic Nanoparticles To Determine Specificity of G-quadruplex Ligands

被引：32

作者：

Panda, Deepanjan ^{[1
]}

Saha, Puja ^{[1
]}

Chaudhuri, Ritapa ^{[1
]}

Prasanth, Thumpati ^{[1
,2
]}

Ravichandiran, Velayutham ^{[2
]}

Dash, Jyotirmayee ^{[1
]}

机构：

[1] Indian Assoc Cultivat Sci, Sch Chem Sci, Kolkata 700032, India

[2] Natl Inst Pharmaceut Educ & Res, Kolkata 700032, India

来源：

ANALYTICAL CHEMISTRY | 2019年 / 91卷 / 12期

关键词：

C-MYC; SMALL-MOLECULE; PROMOTER; BINDING; MITOXANTRONE; TARGET; BCL-2; PROBE; SITE;

D O I：

10.1021/acs.analchem.9b00889

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein, we develop a competitive screening method in which G-quadruplex DNA linked magnetic nanoparticles pull down selective ligands for a particular quadruplex topology from a series of small molecules. The screening strategy is first optimized with known G-quadruplex ligands and then used with a new series of G-quadruplex interactive bis-triazolyl ligands that are synthesized by Cu(I)-catalyzed azide alkyne cycloaddition. The assay enables the identification of c-MYC and BCL2 G-quadruplex selective bis-triazole ligands that specifically target promoter G-quadruplexes in cancer cells.

引用

页码：7705 / 7711

页数：7

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