Influence of Physical Immobilization of dsDNA on Carbon Based Matrices of Electrochemical Sensors

被引：9

作者：

Gugoasa, Livia Alexandra ^{[1
,2
,3
]}

Stefan-van Staden, Raluca-Ioana ^{[1
,2
]}

Ciucu, Anton Alexandru ^{[3
]}

van Staden, Jacobus Frederick ^{[1
,2
]}

机构：

[1] Natl Inst Res Electrochem & Condensed Matter, Lab Electrochem, Bucharest 060021, Romania

[2] Natl Inst Res Electrochem & Condensed Matter, PATLAB Bucharest, Bucharest 060021, Romania

[3] Univ Bucharest, Fac Chem, Dept Analyt Chem, Bucharest, Romania

来源：

CURRENT PHARMACEUTICAL ANALYSIS | 2014年 / 10卷 / 01期

关键词：

dsDNA; sensors; carbon based matrices; neurotransmitters; PASTE-BASED ELECTRODES; DOPAMINE; DNA;

D O I：

10.2174/157341291001140102104740

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The influence of addition of dsDNA on the electrocatalytic effect of carbon matrices, e. g. synthetic monocrystalline diamond, fullerenes C-60 and multi-walled carbon nanotubes was studied. Three neurotransmitters: dopamine, epinephrine and norepinephrine, which are known as biomarkers that play an important role in transporting and modulating nervous impulses in the synapse were chosen as model analytes. Cyclic voltammetry was used to determine the optimum working conditions with a phosphate buffer solution at a pH=4 with KNO3 as support electrolyte. Differential pulse voltammograms (DPVs) were employed to assess the electrolytic effect of dsDNA on carbon matrices under the optimized conditions, and the potential applications of the biosensors for pharmaceutical and biomedical analysis.

引用

页码：20 / 29

页数：10

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