Biosynthesis of DHGA12 and its roles in Arabidopsis seedling establishment

被引：56

作者：

Liu, Hao ^{[1
,2
]}

Guo, Siyi ^{[1
,2
]}

Lu, Minghua ^{[3
]}

Zhang, Yu ^{[1
,2
]}

Li, Junhua ^{[4
]}

Wang, Wei ^{[1
,2
]}

Wang, Pengtao ^{[1
,2
]}

Zhang, Junli ^{[1
,2
]}

Hu, Zhubing ^{[1
,2
]}

Li, Liangliang ^{[1
,2
]}

Si, Lingyu ^{[1
,2
]}

Zhang, Jie ^{[1
,2
]}

Qi, Qi ^{[5
]}

Jiang, Xiangning ^{[5
]}

Botella, Jose Ramon ^{[1
,2
,6
]}

Wang, Hua ^{[7
]}

Song, Chun-Peng ^{[1
,2
]}

机构：

[1] Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, Kaifeng 475004, Peoples R China

[2] Henan Univ, Sch Life Sci, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China

[3] Henan Univ, Coll Chem & Chem Engn, Kaifeng 475004, Peoples R China

[4] Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China

[5] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China

[6] Univ Queensland, Sch Agr & Food Sci, Plant Genet Engn Lab, Brisbane, Qld 4072, Australia

[7] Henan Univ, Engn Res Ctr Nanomat, Kaifeng 475004, Peoples R China

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

中国国家自然科学基金;

关键词：

GIBBERELLIN BIOSYNTHESIS; EXPRESSION; GENE; METABOLISM; THALIANA; DORMANCY; ENCODES; GA4; TRANSCRIPTION; GERMINATION;

D O I：

10.1038/s41467-019-09467-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Seed germination and photoautotrophic establishment are controlled by the antagonistic activity of the phytohormones gibberellins (GAs) and abscisic acid (ABA). Here we show that Arabidopsis thaliana GAS2 (Gain of Function in ABA-modulated Seed Germination 2), a protein belonging to the Fe-dependent 2-oxoglutarate dioxygenase superfamily, catalyzes the stereospecific hydration of GA(12) to produce GA(12) 16, 17-dihydro-16 alpha-ol (DHGA(12)). We show that DHGA(12), a C-20-GA has an atypical structure compared to known active GAs but can bind to the GA receptor (GID1c). DHGA(12) can promote seed germination, hypocotyl elongation and cotyledon greening. Silencing and over-expression of GAS2 alters the ABA/GA ratio and sensitivity to ABA during seed germination and photoautotrophic establishment. Hence, we propose that GAS2 acts to modulate hormonal balance during early seedling development.

引用

页数：14

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