MicroPET Evaluation of a Hydroxamate-Based MMP Inhibitor, [18F]FB-ML5, in a Mouse Model of Cigarette Smoke-Induced Acute Airway Inflammation

被引：5

作者：

Matusiak, Nathalie ^{[1
]}

van Waarde, Aren ^{[1
]}

Rozeveld, Dennie ^{[2
]}

van Oosterhout, Antoon J. M. ^{[2
]}

Heijink, Irene H. ^{[2
]}

Castelli, Riccardo ^{[3
]}

Overkleeft, Herman S. ^{[3
]}

Bischoff, Rainer ^{[4
]}

Dierckx, Rudi A. J. O. ^{[1
]}

Elsinga, Philip H. ^{[1
]}

机构：

[1] Univ Groningen, Univ Med Ctr Groningen, Dept Nucl Med & Mol Imaging, NL-9713 EZ Groningen, Netherlands

[2] Univ Groningen, Univ Med Ctr Groningen, Lab Allergy & Pulm Dis, NL-9713 EZ Groningen, Netherlands

[3] Leiden Univ, Leiden Inst Chem, NL-2300 RA Leiden, Netherlands

[4] Univ Groningen, Groningen Res Inst Pharm, Dept Analyt Biochem, NL-9713 EZ Groningen, Netherlands

来源：

MOLECULAR IMAGING AND BIOLOGY | 2015年 / 17卷 / 05期

关键词：

COPD; MicroPET; Lung imaging; MMP/ADAM inhibitor; BALF; OBSTRUCTIVE PULMONARY-DISEASE; BRONCHOALVEOLAR LAVAGE FLUID; MATRIX METALLOPROTEINASES; TISSUE INHIBITOR; MACROPHAGE ELASTASE; LUNG INFLAMMATION; ASTHMA; MICE; EMPHYSEMA; SPUTUM;

D O I：

10.1007/s11307-015-0847-3

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Matrix metalloproteinases (MMPs) are the main proteolytic enzymes involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). A radiolabeled MMP inhibitor, [F-18]FB-ML5, was prepared, and its in vivo kinetics were tested in a mouse model of pulmonary inflammation. BALB/c mice were exposed for 4 days to cigarette smoke (CS) or air. On the fifth day, a dynamic microPET scan was made with [F-18]FB-ML5. Standardized uptake values (PET-SUVmean) were 0.19 +/- 0.06 in the lungs of CS-exposed mice (n = 6) compared to 0.11 +/- 0.03 (n = 5) in air-exposed controls (p < 0.05), 90 min post-injection MMP-9 levels in bronchoalveolar lavage fluid (BALF) were increased from undetectable level to 4615 +/- 1963 pg/ml by CS exposure. Increased MMP expression in a COPD mouse model was shown to lead to increased retention of [F-18]FB-ML5.

引用

页码：680 / 687

页数：8

共 28 条

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