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Rotation of Escherichia coli F1-ATPase
被引:77
|作者:
Noji, H
Häsler, K
Junge, W
Kinosita, K
Yoshida, M
[1
]
Engelbrecht, S
机构:
[1] Tokyo Inst Technol, Resources Utilizat Res Lab, Yokohama, Kanagawa 2268508, Japan
[2] Keio Univ, Fac Sci & Technol, Yokohama, Kanagawa 2238522, Japan
[3] Univ Osnabruck, Fachbereich Biol Chem, Biophys Abt, D-49069 Osnabruck, Germany
[4] Teikyo Univ, Biotechnol Res Ctr 3F, Genet Programming Team 13, Core Res Evolut Sci & Technol,Miyamae Ku, Kawasaki, Kanagawa 2160001, Japan
关键词:
D O I:
10.1006/bbrc.1999.0885
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
By applying the same method used for F-1-ATPase (TF,) from thermophilic Bacillus PS3 (Noji, H., Yasuda, R., Yoshida, M., and Kinosita, K., Jr. (1997) Nature 386, 299 -302), we observed ATP-driven rotation of a fluorescent actin filament attached to the gamma subunit in Escherichia coli F-1-ATPase. The torque value and the direction of the rotation were the same as those observed for TF1. F-1-ATPases seem to share common properties of rotation irrespective of the sources. (C) 1999 Academic Press.
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页码:597 / 599
页数:3
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