Transcription Factor ZBP-89 Drives a Feedforward Loop of β-Catenin Expression in Colorectal Cancer

被引:24
作者
Essien, Bryan E. [1 ,4 ]
Sundaresan, Sinju [1 ]
Ocadiz-Ruiz, Ramon [1 ]
Chavis, Aaron [1 ]
Tsao, Amy C. [1 ]
Tessier, Arthur J. [1 ]
Hayes, Michael M. [1 ]
Photenhauer, Amanda [1 ]
Saqui-Salces, Milena [1 ,5 ]
Kang, Anthony J. [1 ]
Shah, Yatrik M. [2 ]
Gyorffy, Balazs [3 ]
Merchant, Juanita L. [1 ,2 ]
机构
[1] Univ Michigan, Dept Internal Med Gastroenterol, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 USA
[3] MTA TTK Lendulet Canc Biomarker Res Grp, Budapest, Hungary
[4] Ball State Univ, Dept Biol Anat & Physiol, 2000 W Univ Ave, Muncie, IN 47306 USA
[5] Univ Minnesota, Dept Anim Sci, 495K AS AM,1988 Fitch Ave, Minneapolis, MN USA
关键词
EPITHELIAL-MESENCHYMAL TRANSITION; GENE-EXPRESSION; TARGET GENES; IN-VITRO; CARCINOMA; COLON; CELLS; PROMOTER; DIFFERENTIATION; PROGRESSION;
D O I
10.1158/0008-5472.CAN-15-3150
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
In colorectal cancer, APC-mediated induction of unregulated cell growth involves posttranslational mechanisms that prevent proteasomal degradation of proto-oncogene beta-catenin (CTNNB1) and its eventual translocation to the nucleus. However, about 10% of colorectal tumors also exhibit increased CTNNB1 mRNA. Here, we show in colorectal cancer that increased expression of ZNF148, the gene coding for transcription factor ZBP-89, correlated with reduced patient survival. Tissue arrays showed that ZBP-89 protein was overexpressed in the early stages of colorectal cancer. Conditional deletion of Zfp148 in a mouse model of Apc-mediated intestinal polyps demonstrated that ZBP-89 was required for polyp formation due to induction of Ctnnb1 gene expression. Chromatin immunoprecipitation (ChIP) and EMSA identified a ZBP-89-binding site in the proximal promoter of CTNNB1. Reciprocally, siRNA-mediated reduction of CTNNB1 expression also decreased ZBP-89 protein. ChIP identified TCF DNA binding sites in the ZNF148 promoter through which Wnt signaling regulates ZNF148 gene expression. Suppression of either ZNF148 or CTNNB1 reduced colony formation in WNT-dependent, but not WNT-independent cell lines. Therefore, the increase in intracellular beta-catenin protein initiated by APC mutations is sustained by ZBP-89-mediated feedforward induction of CTNNB1 mRNA. (C) 2016 AACR.
引用
收藏
页码:6877 / 6887
页数:11
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