Structural elucidation of a novel water-soluble fructan isolated from Wedelia prostrata

被引：12

作者：

Zhang, An-qiang ^{[1
]}

Zhang, Yang ^{[1
]}

Yang, Jun ^{[2
]}

Jiang, Jian ^{[2
]}

Huang, Fang-fang ^{[2
]}

Sun, Pei-long ^{[1
]}

机构：

[1] Zhejiang Univ Technol, Coll Biol & Environm Engn, Hangzhou 310014, Zhejiang, Peoples R China

[2] China Tobacco Zhejiang Ind Co Ltd, Hangzhou 310014, Zhejiang, Peoples R China

来源：

CARBOHYDRATE RESEARCH | 2013年 / 376卷

关键词：

Wedelia prostrata; Fructan; Structural elucidation; NMR analysis;

D O I：

10.1016/j.carres.2013.04.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new heteropolysaccharide, WPP60A, with a molecular weight of 2.4 kDa, was isolated from Wedelia prostrata by hot water extraction, and purified by DEAE Sepharose fast flow and Sephacryl S-200 high-resolution chromatography. Compositional analysis, and methylation analysis, combined with H-1, C-13 NMR spectroscopy including 2D NMR (COSY, TOCSY, HMQC, NOESY, and HMBC) experiments demonstrated that WPP60A was composed of primarily fructose and low glucose. Results indicated that this new heteropolysaccharide consists of a repeating unit with the following structure: [GRAPHICS] (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：24 / 28

页数：5

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