Human embryo;
Vitrification;
Re-vitrification;
Transfer;
Birth;
VITRIFICATION METHOD;
CRYOPRESERVATION;
D O I:
10.1016/j.cryobiol.2012.05.012
中图分类号:
Q [生物科学];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
The role of cryopreservation in assisted reproductive technology programs has increased within the last years allowing the transfer of a limited number of embryos and the storage of the remaining for future use. The reduction in the number of transferred embryos decreases the frequency of multiple pregnancy rates and of ovarian hyperstimulation syndrome while the cumulative pregnancy rate can be maximized. Moreover, as not all embryos will survive the warming process more cleavage stage embryos are warmed to improve selection for transfer. Therefore, surplus good quality cleavage stage embryos and/or blastocysts must be re-vitrified for further transfer to achieve pregnancy. To our knowledge, there have been no reports demonstrating that human embryos can be successfully vitrified/warmed twice at the cleavage stage. Thus we report two successful pregnancies and deliveries of healthy babies after transfer of embryos that were twice vitrified/warmed at 2-4 cells stage. (C) 2012 Elsevier Inc. All rights reserved.
机构:
IVF Wales, Assisted Reprod, Cardiff CF14 4XN, S Glam, Wales
Cardiff Univ, Univ Wales Hosp, Cardiff, S Glam, WalesIVF Wales, Assisted Reprod, Cardiff CF14 4XN, S Glam, Wales
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Loutradi, Kalliopi E.
;
Kolibianakis, Efstratios M.
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Kolibianakis, Efstratios M.
;
Venetis, Christos A.
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Venetis, Christos A.
;
Papanikolaou, Evangelos G.
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Papanikolaou, Evangelos G.
;
Pados, George
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Pados, George
;
Bontis, Ioannis
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Bontis, Ioannis
;
Tarlatzis, Basil C.
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
机构:
IVF Wales, Assisted Reprod, Cardiff CF14 4XN, S Glam, Wales
Cardiff Univ, Univ Wales Hosp, Cardiff, S Glam, WalesIVF Wales, Assisted Reprod, Cardiff CF14 4XN, S Glam, Wales
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Loutradi, Kalliopi E.
;
Kolibianakis, Efstratios M.
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Kolibianakis, Efstratios M.
;
Venetis, Christos A.
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Venetis, Christos A.
;
Papanikolaou, Evangelos G.
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Papanikolaou, Evangelos G.
;
Pados, George
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Pados, George
;
Bontis, Ioannis
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece
Bontis, Ioannis
;
Tarlatzis, Basil C.
论文数: 0引用数: 0
h-index: 0
机构:
Papageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, GreecePapageorgiou Gen Hosp, Dept Obstet & Gynecol 1, Human Reprod Unit, Thessaloniki 54603, Greece