Structure of an Xrcc4-DNA ligase IV yeast ortholog complex reveals a novel BRCT interaction mode

被引：52
作者：
Doré, AS
Furnham, N
Davies, OR
Sibanda, BL
Chirgadze, DY
Jackson, SP
Pellegrini, L
Blundell, TL
机构：
[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England
[2] Univ Cambridge, Dept Zool, Cambridge CB2 1QR, England
[3] Univ Cambridge, Gurdon Inst, Wellcome Trust & Canc UK, Cambridge CB2 1QR, England
来源：
DNA REPAIR | 2006年 / 5卷 / 03期
基金：
英国惠康基金;
关键词：
DNA repair; Xrcc4; DNA ligase IV; BRCT; coiled-coil;
D O I：
10.1016/j.dnarep.2005.11.004
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
DNA ligase IV catalyses the final ligation step in the non-homologous end-joining (NHEJ) DNA repair pathway and requires interaction of the ligase with the Xrcc4'genome-guardian', an essential NHEJ factor. Here we report the 3.9 angstrom crystal structure of the Saccharomyces cerevisiae Xrcc4 ortholog ligase interacting factor 1 (Lif1p) complexed with the C-terminal BRCT domains of DNA ligase IV (Lig4p). The structure reveals a novel mode of protein recognition by a tandem BRCT repeat, and in addition provides a molecular basis for a human LIG4 syndrome clinical condition. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：362 / 368
页数：7
相关论文
共 27 条
[1]   Methods used in the structure determination of bovine mitochondrial F-1 ATPase [J].
Abrahams, JP ;
Leslie, AGW .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1996, 52 :30-42
[2]  
[Anonymous], [No title captured]
[3]   Low-resolution crystallography is coming of age [J].
Brunger, AT .
STRUCTURE, 2005, 13 (02) :171-172
[4]  
Brunger AT, 1998, ACTA CRYSTALLOGR D, V54, P905, DOI 10.1107/s0907444998003254
[5]   Structure and mechanism of BRCA1 BRCT domain recognition of phosphorylated BACH1 with implications for cancer [J].
Clapperton, JA ;
Manke, IA ;
Lowery, DM ;
Ho, T ;
Haire, LF ;
Yaffe, MB ;
Smerdon, SJ .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (06) :512-518
[6]   Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV [J].
Critchlow, SE ;
Bowater, RP ;
Jackson, SP .
CURRENT BIOLOGY, 1997, 7 (08) :588-598
[7]  
DeLano W.L., 2002, DELANO SCI
[8]   Heterogeneity and inaccuracy in protein structures solved by X-ray crystallography [J].
DePristo, MA ;
de Bakker, PIW ;
Blundell, TL .
STRUCTURE, 2004, 12 (05) :831-838
[9]   Crystal structure of human 53BP1 BRCT domains bound to p53 tumour suppressor [J].
Derbyshire, DJ ;
Basu, BP ;
Serpell, LC ;
Joo, WS ;
Date, T ;
Iwabuchi, K ;
Doherty, AJ .
EMBO JOURNAL, 2002, 21 (14) :3863-3872
[10]   Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells [J].
Grawunder, U ;
Wilm, M ;
Wu, XT ;
Kulesza, P ;
Wilson, TE ;
Mann, M ;
Lieber, MR .
NATURE, 1997, 388 (6641) :492-495
123