Electrochemical Sensor for the Detection of Riboflavin Based on Nanocomposite Film of Polydeoxyadenylic Acid/Reduced Graphene Oxide

A novel electrochemical sensor to detect riboflavin was constructed using 32-mer polydeoxyadenylic acid(A(32)) as an effective adhesive for binding with the hydrazine hydrate-reduced graphene oxide(RGO) and the gold(Au) electrode. The fabrication process of the sensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy, and its electrochemical behaviour was studied by cyclic voltammetry and differential pulse voltammetry. The experimental results showed that compared with the bare Au electrode and the A(32)/Au electrode, the RGO/A(32)/Au electrode displayed excellent electrochemical activity towards riboflavin, and the cathodic peak current increased linearly with the increasing concentration of riboflavin from 0. 025 mu mol/L to 2. 75 mu mol/L with a low detection limit of 15 nmol/L(S/N =3). The sensor possesses high sensitivity, strong anti-interference ability and good stability, and can be used for the analysis of riboflavin in human urine samples.