Synthesis and Characterization of the Gold-SiO2 Core-Shell Nanoparticle on the X-Nanozeoliate used for Immobilization of the Alkaline Protease Enzyme

Immobilized enzymes enhance process robustness, allow longer duration of activity of enzymes, and re-use of the same enzymes in multiple cycles. Enzymes can be operated in the liquid form or immobilized on various supports. In this work, we prepared gold nanoparticle core-shell structure by assembling of the gold nano particles on the surface of amine-functionalized x-type zeolite and then used them for immobilization of the alkaline protease. Characterization of these assembled systems were carried out by UV-visible, transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy dispersive analysis of X-ray (EDAX). Biocatalytic activity of the alkaline protease in this bioconjugate system was examined and showed an increase in comparing with the free enzyme in solution.