Evaluation of Capsule Permeability in the Equine Blastocyst

被引：6

作者：

Scott, Brittany R. ^{[1
]}

Carwell, David B. ^{[1
]}

Hill, Rebecca A. ^{[1
]}

Bondioli, Kenneth R. ^{[1
]}

Godke, Robert A. ^{[1
]}

Gentry, Glen T. ^{[1
]}

机构：

[1] Louisiana State Univ, Ctr Agr, Louisiana Agr Expt Stn, Sch Anim Sci, Baton Rouge, LA 70803 USA

来源：

JOURNAL OF EQUINE VETERINARY SCIENCE | 2012年 / 32卷 / 12期

关键词：

Equine; Embryo; Capsule; Permeability; Glycerol; Cryopreservation; PREGNANCY RATES; EMBRYONIC LOSS; FISH EMBRYOS; CRYOPRESERVATION; VIABILITY; GLYCEROL; MARES;

D O I：

10.1016/j.jevs.2012.03.010

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Recipient pregnancy rates following transfer of frozen-thawed blastocyst stage equine embryos remain low. To date, no protocol has been developed that would allow successful cryopreservation of this stage of embryos. These experiments characterized the amount of glycerol entering equine embryos after incubation in 1.4 M and 3.4 M glycerol solutions using tritiated glycerol and a liquid scintillation counter. Blastocyst stage equine embryos (n = 27) were collected and incubated for 15 minutes in either 1.4 M (n = 14) or 3.4 M (n = 13) tritiated glycerol solutions. Disintegrations per minute were then determined, and the percent glycerol uptake was calculated for each embryo. Percent glycerol uptake for 1.4 M or 3.4 M glycerol treatment groups was not different (P = .68). However, it was higher (P = .05) in embryos with a diameter of <= 600 mu m (3.6%) compared with embryos with a diameter of >600 mu m (0.4%). We concluded that glycerol more readily permeates into embryos with a diameter of <= 600 mu m that do not possess a fully functional capsule compared with embryos with a diameter of >600 mu m with a fully formed capsule. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：795 / 798

页数：4

共 31 条

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