Direct PCR amplification from leaf discs

被引：22
作者：
Rogers, HJ
Parkes, HC
机构：
[1] Univ Wales Coll Cardiff, Sch Biosci, Cardiff CF1 3TL, S Glam, Wales
[2] Lab Govt Chemist, Teddington TW11 0LY, Middx, England
来源：
PLANT SCIENCE | 1999年 / 143卷 / 02期
关键词：
PCR from leaves; multiplex PCR; RAPDs; transgenic screening;
D O I：
10.1016/S0168-9452(99)00048-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
PCR amplification from leaf tissue has become an integral part of many plant molecular biology applications including screening for transformants, plant breeding, and molecular ecology. We have adapted an existing method to produce a simple protocol for the amplification of single or multicopy genes directly from leaf discs, particularly useful when screening large numbers of individuals. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：183 / 186
页数：4
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