Nanomaterial-Based Fluorescent DNA Analysis: A Comparative Study of the Quenching Effects of Graphene Oxide, Carbon Nanotubes, and Gold Nanoparticles

被引:173
作者
Li, Fan [1 ]
Pei, Hao [1 ]
Wang, Lihua [1 ]
Lu, Jianxin [2 ]
Gao, Jimin [2 ]
Jiang, Bowei [3 ]
Zhao, Xingchun [3 ]
Fan, Chunhai [1 ]
机构
[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China
[2] Wenzhou Med Coll, Minist Educ, Key Lab Lab Med, Wenzhou 325035, Zhejiang, Peoples R China
[3] Minist Publ Secur, Inst Forens Sci, Beijing 100038, Peoples R China
关键词
graphene oxide; gold nanoparticles; carbon nanotubes; DNA; fluorescence; SEQUENCE-SPECIFIC DETECTION; MOLECULAR BEACONS; SURFACE SCIENCE; PROBES; HYBRIDIZATION; ADSORPTION; FUNCTIONALIZATION; IMMOBILIZATION; RECOGNITION; DERIVATIVES;
D O I
10.1002/adfm.201203816
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A variety of nanomaterials have shown extraordinarily high quenching ability toward a broad range of fluorophores. Recently, there has been intense interest in developing new tools for fluorescent DNA analysis in solution or inside the cell based on this property, and by exploiting interactions between these nanoscale superquenchers and DNA molecules in the single-stranded (ss-) or double-stranded (ds-) forms. Here, a comparative study on the nanoqueching effects is performed by using a series of nanomaterials with different dimensions, i.e., gold nanoparticles (AuNPs, 0D), carbon nanotubes (CNTs, 1D), and graphene oxide (GO, 2D). The quenching efficiency, kinetics, differentiation ability, and influencing factors such as concentration and ionic strength are studied. Interestingly, GO exhibits superior quenching abilities to the other two materials in both the quenching efficiency and kinetics. As a result, a GO-based fluorescent sensor, designed in a simple mix-and-detect format, can detect concentrations of DNA as low as 0.2 nM, which is better than either CNTs or AuNPs by an order of magnitude. This sensor can also differentiate single-base mismatches much better than either CNTs- or AuNPs- based sensors. This study paves the way to better choice of nanomaterials for bioanalysis and elaborate design of biosensors for both in vitro diagnosis and in vivo bioimaging.
引用
收藏
页码:4140 / 4148
页数:9
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