Parentage assignment of the mud crab (Scylla paramamosain) based on microsatellite markers

被引：18

作者：

Ma, Qunqun ^{[1
,2
]}

Ma, Hongyu ^{[1
,2
]}

Chen, Jianhua ^{[2
]}

Ma, Chunyan ^{[1
]}

Feng, Nana ^{[1
]}

Xu, Zhen ^{[1
]}

Li, Shujuan ^{[1
]}

Jiang, Wei ^{[1
]}

Qiao, Zhenguo ^{[1
]}

Ma, Lingbo ^{[1
]}

机构：

[1] Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab East China Sea & Ocean Fishery Resources, Minist Agr, Shanghai 200090, Peoples R China

[2] Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2013年 / 49卷

基金：

中国国家自然科学基金;

关键词：

Scylla paramamosain; Microsatellite makers; Parentage assignment; Genetic diversity; FLOUNDER PARALICHTHYS-OLIVACEUS; RELATEDNESS DETERMINATION; DNA MARKERS; POPULATION; ERROR; LOCI;

D O I：

10.1016/j.bse.2013.03.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, microsatellite markers were employed to identify the parentage relationship in Scylla paramamosain. The exclusion probability of loci was found to be related with the level of their heterozygosity. When no parent information or only one parent information was available, the exclusion probability ranged from 22.0% to 56.6% and from 41.2% to 73.1%, with the combined exclusion probability for ten loci being 97.0% and 99.8%, respectively. The cumulative assignment success rate was 100% when no parent information was available using seven most informative microsatellite markers. Moreover, the power of the seven microsatellite markers for parentage assignment was tested by a double-blind test, which indicated that 95% of the progeny can be correctly assigned to their parents. This study provided a microsatellite-based approach for parentage assignment in S. paramamosain that will be useful for investigation of genetic background and molecular marker-assisted selective breeding in this important crab species. (c) 2013 Elsevier Ltd. All rights reserved.

引用

页码：62 / 68

页数：7

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