Estrogen oversees the maintenance of the female genetic program in terminally differentiated gonochorists

被引：85

作者：

Paul-Prasanth, B. ^{[1
,2
]}

Bhandari, R. K. ^{[1
,2
,3
]}

Kobayashi, T. ^{[4
]}

Horiguchi, R. ^{[1
,2
,3
]}

Kobayashi, Y. ^{[1
,2
,3
]}

Nakamoto, M. ^{[1
,2
]}

Shibata, Y. ^{[1
,2
]}

Sakai, F. ^{[1
,2
]}

Nakamura, M. ^{[1
,3
,5
]}

Nagahama, Y. ^{[1
,2
,6
]}

机构：

[1] Natl Inst Nat Sci, Natl Inst Basic Biol, SORST, Okazaki, Aichi 4448585, Japan

[2] Natl Inst Nat Sci, Natl Inst Basic Biol, Lab Reprod Biol, Okazaki, Aichi 4448585, Japan

[3] Univ Ryukyus, Trop Biosphere Res Ctr, Sesoko Stn, Motobu, Okinawa 9050227, Japan

[4] Univ Shizuoka, Grad Sch Integrated Pharmaceut & Nutr Sci, Lab Mol Reprod Biol, Shizuoka 4228526, Japan

[5] Okinawa Churashima Fdn, Motobu, Okinawa 9050206, Japan

[6] Ehime Univ, South Ehime Fisheries Res Ctr, Matsuyama, Ehime 7908577, Japan

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

基金：

日本科学技术振兴机构;

关键词：

OOGENESIS; STEROID HORMONES; DIFFERENTIATION; CELL LINEAGE; SEX-DETERMINING REGION; GERMLINE STEM-CELLS; AROMATASE INHIBITOR; MEDAKA FISH; EXPRESSION; TELEOST; CYTOCHROME-P450; IDENTIFICATION; GENERATION; REVERSAL;

D O I：

10.1038/srep02862

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The sexual plasticity of the gonads is not retained after the completion of sex differentiation in vertebrates, except in some hermaphroditic species. Here, we report that the depletion of estradiol-17 beta (E-2) by aromatase inhibitors (AI) for up to six months resulted in a functional female-to-male sex reversal in sexually-mature adults of two gonochoristic fish species, Nile tilapia and medaka. The sex-reversed fish showed a typical male pattern of E-2 and androgen levels, secondary sexual characteristics, and male-like sex behavior, producing fertile sperm. Conversely, co-treatment of E-2 inhibited AI-induced sex reversal. In situ hybridization of medaka gonads during AI-induced sex reversal indicated that cysts on the dorsal side of the adult ovaries are the origin of germ cells and Sertoli cells in the newly formed testicular tissue. Gonochoristic fish maintain their sexual plasticity until adulthood and E-2 plays a critical role in maintaining the female phenotype.

引用

页数：10

共 33 条

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