Overexpression of Small Heat Shock Protein LimHSP16.45 in Arabidopsis Enhances Tolerance to Abiotic Stresses

被引：76

作者：

Mu, Changjun ^{[1
]}

Zhang, Shijia ^{[1
]}

Yu, Guanzhong ^{[1
]}

Chen, Ni ^{[1
]}

Li, Xiaofeng ^{[1
]}

Liu, Heng ^{[1
]}

机构：

[1] Lanzhou Univ, Inst Cell Biol, Sch Life Sci, Lanzhou 730000, Peoples R China

来源：

PLOS ONE | 2013年 / 8卷 / 12期

基金：

中国国家自然科学基金;

关键词：

MEIOTIC PROPHASE I; ALPHA-CRYSTALLIN; TRANSGENIC TOBACCO; OXIDATIVE STRESS; PACHYTENE STAGES; LATE ZYGOTENE; EXPRESSION; SUBSTRATE; GRANULES; CADMIUM;

D O I：

10.1371/journal.pone.0082264

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Small heat shock proteins (smHSPs) play important and extensive roles in plant defenses against abiotic stresses. We cloned a gene for a smHSP from the David Lily (Lilium davidii (E. H. Wilson) Raffill var. Willmottiae), which we named LimHSP16.45 based on its protein molecular weight. Its expression was induced by many kinds of abiotic stresses in both the lily and transgenic plants of Arabidopsis. Heterologous expression enhanced cell viability of the latter under high temperatures, high salt, and oxidative stress, and heat shock granules (HSGs) formed under heat or salinity treatment. Assays of enzymes showed that LimHSP16.45 overexpression was related to greater activity by superoxide dismutase and catalase in transgenic lines. Therefore, we conclude that heterologous expression can protect plants against abiotic stresses by preventing irreversible protein aggregation, and by scavenging cellular reactive oxygen species.

引用

页数：9

共 47 条

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