Simple in vitro translation assay to analyze inhibitors of rhinovirus proteases

被引：18

作者：

Heinz, BA

Tang, J

Labus, JM

Chadwell, FW

Kaldor, SW

Hammond, M

机构：

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1996年 / 40卷 / 01期

关键词：

D O I：

10.1128/AAC.40.1.267

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have developed a simple in vitro translation method to analyze compounds that inhibit the rhinovirus 3C protease in peptide substrate assays but demonstrate no antiviral activity. This complementary assay, which provides both qualitative and quantitative results, detects the inhibition of the 3CD protease in the native polyprotein form.

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页码：267 / 270

页数：4

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