CRISPR-Cas-mediated gene editing in lactic acid bacteria

被引：14

作者：

Song, Xin ^{[1
]}

Zhang, Xiao-yu ^{[1
]}

Xiong, Zhi-qiang ^{[1
]}

Liu, Xin-xin ^{[1
]}

Xia, Yong-jun ^{[1
]}

Wang, Shi-jie ^{[2
,3
]}

Ai, Lian-zhong ^{[1
]}

机构：

[1] Univ Shanghai Sci & Technol, Shanghai Engn Res Ctr Food Microbiol, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China

[2] Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shijiazhuang 050018, Hebei, Peoples R China

[3] Shijiazhuang Junlebao Dairy Co Ltd, Shijiazhuang 050211, Hebei, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2020年 / 47卷 / 10期

关键词：

CRISPR; Lactic acid bacteria; Gene editing; Gene knockout; LACTOBACILLUS-ACIDOPHILUS; LACTOCOCCUS-LACTIS; REPLACEMENT SYSTEM; DNA CLEAVAGE; GENOME; RECOMBINATION; INTEGRATION; EXPRESSION; IDENTIFICATION; MUTAGENESIS;

D O I：

10.1007/s11033-020-05820-w

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The high efficiency, convenience and diversity of clustered regular interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are driving a technological revolution in the gene editing of lactic acid bacteria (LAB). Cas-RNA cassettes have been adopted as tools to perform gene deletion, insertion and point mutation in several species of LAB. In this article, we describe the basic mechanisms of the CRISPR-Cas system, and the current gene editing methods available, focusing on the CRISPR-Cas models developed for LAB. We also compare the different types of CRISPR-Cas-based genomic manipulations classified according to the different Cas proteins and the type of recombineering, and discuss the rapidly evolving landscape of CRISPR-Cas application in LAB.

引用

页码：8133 / 8144

页数：12

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