Demineralizing potential of dental biofilm added with Candida albicans and Candida parapsilosis isolated from preschool children with and without caries

被引：10
作者：
de Abreu Brandi, Thayse Caroline [1 ]
Portela, Maristela Barbosa [2 ]
Lima, Paula Moraes [1 ]
Barbosa de Araujo Castro, Gloria Fernanda [1 ]
Maia, Lucianne Cople [1 ]
Fonseca-Goncalves, Andrea [1 ]
机构：
[1] Univ Fed Rio de Janeiro, Fac Odontol, Dept Odontopediat & Ortodontia, BR-21941 Rio De Janeiro, Brazil
[2] Univ Fed Fluminense, Fac Odontol, Dept Clin & Odontopediat, Niteroi, RJ, Brazil
来源：
MICROBIAL PATHOGENESIS | 2016年 / 100卷
关键词：
Microhardness; Dental caries; Dental plaque; Children; Candida albicans; Candida parapsilosis; GLUCOSYLTRANSFERASE B; STREPTOCOCCUS-MUTANS; DIVERSITY;
D O I：
10.1016/j.micpath.2016.09.003
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
This study aimed to investigate the demineralizing potential of dental biofilm added of Candida albicans (CA) and Candida parapsilosis (CP), isolated from preschoolers with and without caries. Bovine enamel blocks (n = 48), with initial hardness = 341.50 +/- 21,83 kg/mm(2) were fixed in 24 well plates containing culture media. A pool of children saliva (PHS) was the inoculum for biofilm formation in the presence or absence of isolated CA or CP in accordance with each group (G n = 8): G1 - PHS; G2 - PHS + CA isolated from children with caries; G3 - PHS + CP isolated from children with caries; G4 - PHS + CA isolated from children without caries; G5 - PHS + CP isolated from children without caries; and G6 - blank control. The plates were incubated at 37 degrees C for 5 days, with daily changes of culture media. The microhardness loss percentage (MHL%) of the blocks was calculated, taking in account the hardness values before and after the experiment. Dental biofilm became more cariogenic, independently of the isolated Candida species. The highest MHL% was observed in G4 (85.90 +/- 8.72%) and G5 (86.13 +/- 6.74%) compared to the others (p < 0.001): G1 (34.30 +/- 14,30%) < G2 (59.40 +/- 10.56%) and G3 (65.80 +/- 6.36%) < G6 (13.68 +/- 4.86%) (p < 0.001). C. albicans and C parapsilosis isolates induced the demineralization of the dental enamel. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：51 / 55
页数：5
相关论文
共 25 条
[1]   Microcosm Biofilms Originating from Children with Different Caries Experience Have Similar Cariogenicity under Successive Sucrose Challenges [J].
Azevedo, M. S. ;
van de Sande, F. H. ;
Romano, A. R. ;
Cenci, M. S. .
CARIES RESEARCH, 2011, 45 (06) :510-517
[2]   The oral metagenome in health and disease [J].
Belda-Ferre, Pedro ;
Alcaraz, Luis David ;
Cabrera-Rubio, Raul ;
Romero, Hector ;
Simon-Soro, Aurea ;
Pignatelli, Miguel ;
Mira, Alex .
ISME JOURNAL, 2012, 6 (01) :46-56
[3]   Resins-based denture soft lining materials modified by chlorhexidine salt incorporation: An in vitro analysis of antifungal activity, drug release and hardness [J].
Bertolini, Martinna M. ;
Portela, Maristela B. ;
Curvelo, Jose Alexandre R. ;
Soares, Rosangela M. A. ;
Lourenco, Eduardo J. V. ;
Telles, Daniel M. .
DENTAL MATERIALS, 2014, 30 (08) :793-798
[4]   Biofilm of Candida albicans from oral cavity of an HIV-infected child: challenge on enamel microhardness [J].
Charone, Senda ;
Portela, Maristela Barbosa ;
das Chagas, Madeleine Souza ;
de Araujo Soares, Rosangela Maria ;
Barbosa de Araujo Castro, Gloria Fernanda .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, 2013, 115 (04) :500-504
[5]  
Papa AMCD, 2010, PEDIATR DENT, V32, P35
[6]   Role of Glucosyltransferase B in Interactions of Candida albicans with Streptococcus mutans and with an Experimental Pellicle on Hydroxyapatite Surfaces [J].
Gregoire, S. ;
Xiao, J. ;
Silva, B. B. ;
Gonzalez, I. ;
Agidi, P. S. ;
Klein, M. I. ;
Ambatipudi, K. S. ;
Rosalen, P. L. ;
Bauserman, R. ;
Waugh, R. E. ;
Koo, H. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (18) :6357-6367
[7]   Comparative Phenotypic Analysis of the Major Fungal Pathogens Candida parapsilosis and Candida albicans [J].
Holland, Linda M. ;
Schroeder, Markus S. ;
Turner, Siobhan A. ;
Taff, Heather ;
Andes, David ;
Grozer, Zsuzsanna ;
Gacser, Attila ;
Ames, Lauren ;
Haynes, Ken ;
Higgins, Desmond G. ;
Butler, Geraldine .
PLOS PATHOGENS, 2014, 10 (09)
[8]   Binding Force Dynamics of Streptococcus mutans-glucosyltransferase B to Candida albicans [J].
Hwang, G. ;
Marsh, G. ;
Gao, L. ;
Waugh, R. ;
Koo, H. .
JOURNAL OF DENTAL RESEARCH, 2015, 94 (09) :1310-1317
[9]   Prevalence of Candida species in Turkish children:: relationship between dietary intake and carriage [J].
Kadir, T ;
Uygun, B ;
Akyüz, S .
ARCHIVES OF ORAL BIOLOGY, 2005, 50 (01) :33-37
[10]   Diversity of Lactobacillus species in deep carious lesions of primary molars. [J].
Kneist S. ;
Schmidt F. ;
Callaway A. ;
Willershausen B. ;
Rupf S. ;
Wicht M. ;
Thiede B. .
European Archives of Paediatric Dentistry, 2010, 11 (4) :181-186
123