Vasoactive Intestinal Peptide (VIP) Protects Nile Tilapia (Oreochromis niloticus) against Streptococcus agalatiae Infection

被引：8
作者：
Zhang, Zhiqiang [1 ]
Li, Qi [1 ]
Huang, Yongxiong [1 ]
Xu, Zhou [1 ]
Chen, Xinjin [1 ]
Jiang, Baijian [1 ]
Huang, Yu [1 ,2 ,3 ]
Jian, Jichang [1 ,2 ,3 ]
机构：
[1] Guangdong Ocean Univ, Coll Fishery, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
[3] Aquat Anim Hlth Assessment, Guangdong Prov Engn Res Ctr, Shenzhen 327005, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 23期
基金：
中国国家自然科学基金;
关键词：
Nile tilapia; Streptococcus agalatiae; vasoactive intestinal peptide (VIP); vasoactive intestinal polypeptide receptor 1 (VIPR1); immune response; fish; CYCLASE-ACTIVATING POLYPEPTIDE; FUNCTIONAL EXPRESSION; RECEPTORS; MICE; PHARMACOLOGY; SECRETION; INHIBIT; IDENTIFICATION; INFLAMMASOME; TOLERANCE;
D O I：
10.3390/ijms232314895
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Vasoactive intestinal peptide (VIP), a member of secretin/glucagon family, is involved in a variety of biological activities such as gut motility, immune responses, and carcinogenesis. In this study, the VIP precursor gene (On-VIP) and its receptor gene VIPR1 (On-VIPR1) were identified from Nile tilapia (Oreochromis niloticus), and the functions of On-VIP in the immunomodulation of Nile tilapia against bacterial infection were investigated and characterized. On-VIP and On-VIPR1 contain a 450 bp and a 1326 bp open reading frame encoding deduced protein of 149 and 441 amino acids, respectively. Simultaneously, the transcript of both On-VIP and On-VIPR1 were highly expressed in the intestine and sharply induced by Streptococcus agalatiae. Moreover, the positive signals of On-VIP and On-VIPR1 were detected in the longitudinal muscle layer and mucosal epithelium of intestine, respectively. Furthermore, both in vitro and in vivo experiments indicated several immune functions of On-VIP, including reduction of P65, P38, MyD88, STAT3, and AP1, upregulation of CREB and CBP, and suppression of inflammation. Additionally, in vivo experiments proved that On-VIP could protect Nile tilapia from bacterial infection and promote apoptosis and pyroptosis. These data lay a theoretical basis for further understanding of the mechanism of VIP guarding bony fish against bacterial infection.
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页数：22
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