Bright photoactivatable fluorophores for single-molecule imaging

被引：4

作者：

Grimm, Jonathan B. ^{[1
]}

English, Brian P. ^{[1
]}

Choi, Heejun ^{[1
]}

Muthusamy, Anand K. ^{[1
]}

Mehl, Brian P. ^{[1
]}

Dong, Peng ^{[1
]}

Brown, Timothy A. ^{[1
]}

Lippincott-Schwartz, Jennifer ^{[1
]}

Liu, Zhe ^{[1
]}

Lionnet, Timothee ^{[1
]}

Lavis, Luke D. ^{[1
]}

机构：

[1] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA 20147 USA

来源：

NATURE METHODS | 2016年 / 13卷 / 12期

关键词：

SUPERRESOLUTION MICROSCOPY; LOCALIZATION MICROSCOPY; FLUORESCENT PROTEINS; CHEMICAL BIOLOGY; GENERAL-METHOD; IN-VIVO; RHODAMINE; CELLS; PHOTOLYSIS; SOFTWARE;

D O I：

10.1038/NMETH.4034

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.

引用

页码：985 / +

页数：7

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