Effects of methotrexate on inflammatory alterations induced by obesity: An in vivo and in vitro study

被引：26

作者：

DeOliveira, Caroline Candida ^{[1
]}

Acedo, Simone Coghetto ^{[1
]}

Ferreira Gotardo, Erica Martins ^{[1
]}

Carvalho, Patricia de Oliveira ^{[1
]}

Rocha, Thalita ^{[1
]}

Pedrazzoli, Jose, Jr. ^{[1
]}

Gambero, Alessandra ^{[1
]}

机构：

[1] Univ Sao Francisco, Sch Med, Clin Pharmacol & Gastroenterol Unit, BR-12916900 Braganca Paulista, SP, Brazil

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2012年 / 361卷 / 1-2期

基金：

巴西圣保罗研究基金会;

关键词：

Adipokines; Macrophages; iNOS; Adipose tissue; Adipocytes; JNK; NECROSIS-FACTOR-ALPHA; ADIPOSE-TISSUE; ADENOSINE A(2A); EXPRESSION; RECEPTOR; INTERLEUKIN-6; SUPPRESSION; MECHANISMS; DIET;

D O I：

10.1016/j.mce.2012.03.016

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Immunosuppressant drugs, such as methotrexate, are able to inhibit cytokine production and leukocyte migration to inflammatory foci; therefore, they could modify the establishment of inflammation in adipose tissue during obesity. Thus, we studied the effects of methotrexate in vivo on high-fat diet induced-obesity in mice and in vitro in isolated and co-cultured adipocytes and macrophages. Obese mice treated with methotrexate presented reduced serum levels of TNF-alpha, insulin and glucose, and an improvement of insulin sensitivity. Adipose tissue from these mice produced less proinflammatory (TNF-alpha, IL-6, leptin) and more anti-inflammatory adipokines (adiponectin and IL-10) associated with reduced macrophage infiltration and inflammation. Cytokine inhibition was also confirmed in isolated and co-cultured adipocytes and macrophages. Methotrexate presented anti-lipolytic effect in vivo and, in vitro through adenosine release. Drugs that combine anti-lipolytic effect and the ability to control inflammation in adipose tissue could play a role in the control of insulin resistance and other pathologies associated with obesity. (c) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：92 / 98

页数：7

共 25 条

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