Hyperbranched Glycopolymers for Blood Biocompatibility

被引：64

作者：

Ahmed, Marya ^{[1
]}

Lai, Benjamin F. L. ^{[2
,3
]}

Kizhakkedathu, Jayachandran N. ^{[2
,3
,4
]}

Narain, Ravin ^{[1
]}

机构：

[1] Univ Alberta, Alberta Glyc Ctr, Dept Chem & Mat Engn, Edmonton, AB, Canada

[2] Univ British Columbia, Dept Pathol & Lab Med, Vancouver, BC V5Z 1M9, Canada

[3] Univ British Columbia, Ctr Blood Res, Vancouver, BC V5Z 1M9, Canada

[4] Univ British Columbia, Dept Chem, Vancouver, BC, Canada

来源：

BIOCONJUGATE CHEMISTRY | 2012年 / 23卷 / 05期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

IN-VITRO; POLYMER MATERIALS; MOLECULAR-WEIGHT; COMPLEMENT; POLYGLYCEROL; COPOLYMERS; COMPATIBILITY; BIOMATERIALS; COAGULATION;

D O I：

10.1021/bc3000723

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbohydrate-based drug and gene delivery carriers are becoming extremely popular for in vitro and in vivo applications. These carriers are found to be nontoxic and can play a significant role in targeted delivery. However, the interactions of these carriers with blood cells and plasma components are not well explored. To the best of our knowledge, there are currently no reports that explore the role of carbohydrate based carriers for blood biocompatibility. Hyperbranched glycopolymers of varying molecular weights are synthesized by reversible addition fragmentation chain transfer polymerization (RAFT) and are studied in detail for their biocompatibility, including hemocompatibility and cytotoxicity against different cell lines in vitro. The hemocompatibility studies (such as hemolysis and platelet activation) indicate that hyperbranched glycopolymers of varying molecular weights produced are highly hemocompatible and do not induce clot formation, red blood cell aggregation, and immune response. Hence, it can be concluded that glycopolymers functionalized carriers can serve as an excellent candidate for various biomedical applications. In addition, cytotoxicity of these hyperbranched polymers is studied in primary and malignant cell lines at varying concentrations using cell viability assay.

引用

页码：1050 / 1058

页数：9

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