Complexation of DNA with cationic surfactant

被引:16
|
作者
Kuhn, PS [1 ]
Barbosa, MC [1 ]
Levin, Y [1 ]
机构
[1] Univ Fed Rio Grande do Sul, Inst Fis, BR-91501970 Porto Alegre, RS, Brazil
关键词
thermodynamic functions; equations of state; structure of associated liquids; electrolytes; molten salts; macromolecular and polymer solutions; polymer melts; swelling;
D O I
10.1016/S0378-4371(99)00184-3
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
Transfection of an anionic polynucleotide through a negatively charged membrane is an important problem in genetic engineering. The direct association of cationic surfactant to DNA decreases the effective negative charge of the nucleic acid, allowing the DNA-surfactant complex to approach a negatively charged membrane. The paper develops a theory for solutions composed of polyelectrolyte, salt, and ionic surfactant. The theoretical predictions are compared with the experimental measurements. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:278 / 284
页数:7
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