Micellar gradients in size-exclusion simulated moving bed chromatography

被引：6
作者：
Horneman, DA
Ottens, M
Keurentjes, JTF
van der Wielen, LAM
机构：
[1] Delft Univ Technol, Dept Biotechnol, NL-2628 BC Delft, Netherlands
[2] Eindhoven Univ Technol, Proc Dev Grp, NL-5600 MB Eindhoven, Netherlands
来源：
JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1113卷 / 1-2期
关键词：
bioseparation; protein purification; SMB; SEC; surfactants; gradient elution; chromatography;
D O I：
10.1016/j.chroma.2006.01.134
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The selectivity of size-exclusion chromatography (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatography (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatography with the non-ionic surfactant C12E23 as gradient forming solute, and demonstrated by applying it to a relevant chromatographic protein separation problem. (c) 2006 Published by Elsevier B.V.
引用
收藏
页码：130 / 139
页数：10
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