Cryo-TEM studies of DNA and DNA-lipid structures

被引：26

作者：

Alfredsson, V ^{[1
]}

机构：

[1] Lund Univ, SE-22100 Lund, Sweden

来源：

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE | 2005年 / 10卷 / 5-6期

关键词：

cryo-TEM; DNA; oligonucleotide; lipid; lipoplex; transfection;

D O I：

10.1016/j.cocis.2005.09.005

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Studies published between 2001 and 2005 that utilise cryogenic transmission electron microscopy as a tool for investigating solutions containing DNA with or without amphiphiles present are reviewed. DNA or oligonucleotides form complexes with amphiphilic molecules such as lipids, so-called lipoplexes, and the structures and morphologies are excellent objects to study by cryogenic transmission electron microscopy. Recent studies show that this technique is in effective tool for identification of a range of structures such as unilamellar or multilamellar vesicles or dispersed liquid crystalline phases. (c) 2005 Elsevier Ltd. All rights reserved.

引用

收藏

页码：269 / 273

页数：5

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