An Extremely Alkaline Novel Xylanase from a Newly Isolated Streptomyces Strain Cultivated in Corncob Medium

被引:26
作者
Simkhada, Jaya Ram [1 ]
Yoo, Hah-Young [1 ,2 ]
Choi, Yun Hee [1 ]
Kim, Seung Wook [1 ,2 ]
Yoo, Jin Cheol [1 ]
机构
[1] Chosun Univ, Dept Pharm, Kwangju 501759, South Korea
[2] Korea Univ, Dept Chem & Biol Engn, Seoul, South Korea
关键词
Agro-waste; Corncob; Hyperalkaline xylanase; Streptomyces; Xylooligosaccharides production; THERMOSTABLE XYLANASE; STABLE XYLANASE; PURIFICATION; XYLOOLIGOSACCHARIDES; CLONING;
D O I
10.1007/s12010-012-9914-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Streptomyces sp. CS802, recently isolated from Korean soil, produced xylanase in corncob medium. An extracellular xylanase (Xyn802) was purified by a single-step gel filtration and biochemical properties were studied. It showed high activity in extremely alkaline condition with optimum pH at 12.0 and exhibited stability between pH 7.5 and 13.0. It produced xylobiose and xylotriose as the major products from xylan, suggesting its endoxylanase nature. N-terminal amino acid sequences of Xyn802 were ADRNANRD which are significantly different from the reported xylanase. The activity was enhanced by various detergents and a reducing agent and stable in various organic solvents. Xyn802 produced by utilizing corncob, an agro-waste material, might be a novel xylanase based on its peculiar biochemical characteristics, and it can be a suitable candidate for the production of xylooligosaccharides including other useful products.
引用
收藏
页码:2017 / 2027
页数:11
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