Multiplex polymerase chain reaction assay for the detection of minute virus of mice and mouse parvovirus infections in laboratory mice

被引：5

作者：

Wang, K. W. ^{[1
]}

Chueh, L. L. ^{[1
]}

Wang, M. H. ^{[2
]}

Huang, Y. T. ^{[3
]}

Fang, B. H. ^{[4
]}

Chang, C. Y. ^{[5
]}

Fang, M. C. ^{[6
]}

Chou, J. Y. ^{[7
]}

Hsieh, S. C. ^{[8
]}

Wan, C. H. ^{[1
,9
]}

机构：

[1] Natl Taiwan Univ, Grad Inst Vet Med, Taipei 10617, Taiwan

[2] Tzu Chi Univ, Lab Anim Ctr, Hualien, Taiwan

[3] Natl Appl Res Labs, Natl Lab Anim Ctr, Taipei, Taiwan

[4] Kaohsiung Med Univ, Lab Anim Ctr, Chung Ho Mem Hosp, Kaohsiung, Taiwan

[5] Natl Taiwan Univ, Lab Anim Ctr, Coll Med, Taipei 10617, Taiwan

[6] Natl Def Med Ctr, Lab Anim Ctr, Taipei, Taiwan

[7] Natl Hlth Res Inst, Lab Anim Ctr, Miaoli, Miaoli County, Taiwan

[8] Natl Taiwan Univ, Inst Food Sci & Technol, Taipei 10617, Taiwan

[9] Natl Taiwan Univ, Grad Inst Mol & Comparat Pathobiol, Taipei 10617, Taiwan

来源：

LABORATORY ANIMALS | 2013年 / 47卷 / 02期

关键词：

Minute virus of mice; mouse parvovirus; alpha-actin; multiplex PCR; CONTEMPORARY PREVALENCE; ORPHAN PARVOVIRUS; PCR; REJECTION; SAMPLES; AGENTS; CELLS; RATS;

D O I：

10.1177/0023677213478298

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Mouse parvoviruses are among the most prevalent infectious pathogens in contemporary mouse colonies. To improve the efficiency of routine screening for mouse parvovirus infections, a multiplex polymerase chain reaction (PCR) assay targeting the VP gene was developed. The assay detected minute virus of mice (MVM), mouse parvovirus (MPV) and a mouse housekeeping gene (alpha-actin) and was able to specifically detect MVM and MPV at levels as low as 50 copies. Co-infection with the two viruses with up to 200-fold differences in viral concentrations can easily be detected. The multiplex PCR assay developed here could be a useful tool for monitoring mouse health and the viral contamination of biological materials.

引用

页码：116 / 121

页数：6

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