Shaping Durum Wheat for the Future: Gene Expression Analyses and Metabolites Profiling Support the Contribution of BCAT Genes to Drought Stress Response

被引:23
作者
Buffagni, Valentina [1 ]
Vurro, Filippo [2 ]
Janni, Michela [2 ,3 ]
Gulli, Mariolina [1 ]
Keller, Arturo A. [4 ]
Marmiroli, Nelson [1 ,5 ]
机构
[1] Univ Parma, Dept Chem Life Sci & Environm Sustainabil, Parma, Italy
[2] Natl Res Council CNR, Inst Mat Elect & Magnetism IMEM, Parma, Italy
[3] Natl Res Council CNR, Inst Biosci & Bioresources IBBR, Bari, Italy
[4] Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Santa Barbara, CA 93106 USA
[5] CINSA Interuniv Consortium Environm Sci, Parma, Italy
基金
欧盟地平线“2020”;
关键词
branched-chain aminotransferase; durum wheat; drought stress; gene expression; target metabolomics; CHAIN AMINO-ACIDS; PLANT-GROWTH; PHYSIOLOGICAL-RESPONSES; PROTEIN-DEGRADATION; MOLECULAR ANALYSIS; HORDEUM-VULGARE; ACCUMULATION; TOLERANCE; ARABIDOPSIS; GENOME;
D O I
10.3389/fpls.2020.00891
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Global climate change, its implications for agriculture, and the complex scenario presented by the scientific community are of worldwide concern. Drought is a major abiotic stress that can restrict plants growth and yields, thus the identification of genotypes with higher adaptability to drought stress represents one of the primary goals in breeding programs. During abiotic stress, metabolic adaptation is crucial for stress tolerance, and accumulation of specific amino acids and/or as secondary metabolites deriving from amino acid metabolism may correlate with the increased tolerance to adverse environmental conditions. This work, focused on the metabolism of branched chain-amino acids (BCAAs) in durum wheat and the role of branched-chain amino acid aminotransferases (BCATs) in stress response. The role of BCATs in plant response to drought was previously proposed forArabidopsis, where the levels of BCAAs were altered at the transcriptional level under drought conditions, triggering the onset of defense response metabolism. However, in wheat the role of BCAAs as a trigger of the onset of the drought defense response has not been elucidated. A comparative genomic approach elucidated the composition of the BCAT gene family in durum wheat. Here we demonstrate a tissue and developmental stage specificity of BCATs regulation in the drought response. Moreover, a metabolites profiling was performed on two contrasting durum wheat cultivars Colosseo and Cappelli resulting in the detection of a specific pattern of metabolites accumulated among genotypes and, in particular, in an enhanced BCAAs accumulation in the tolerant cv Cappelli further supporting a role of BCAAs in the drought defense response. The results support the use of gene expression and target metabolomic in modern breeding to shape new cultivars more resilient to a changing climate.
引用
收藏
页数:16
相关论文
共 95 条
[1]   Genomics Approaches for Crop Improvement against Abiotic Stress [J].
Akpinar, Bala Ani ;
Lucas, Stuart J. ;
Budak, Hikmet .
SCIENTIFIC WORLD JOURNAL, 2013,
[2]   Sugarcane Omics: An Update on the Current Status of Research and Crop Improvement [J].
Ali, Ahmad ;
Khan, Mehran ;
Sharif, Rahat ;
Mujtaba, Muhammad ;
Gao, San-Ji .
PLANTS-BASEL, 2019, 8 (09)
[3]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[4]   Genome-Wide Analysis of Branched-Chain Amino Acid Levels in Arabidopsis Seeds [J].
Angelovici, Ruthie ;
Lipka, Alexander E. ;
Deason, Nicholas ;
Gonzalez-Jorge, Sabrina ;
Lin, Haining ;
Cepela, Jason ;
Buell, Robin ;
Gore, Michael A. ;
DellaPenna, Dean .
PLANT CELL, 2013, 25 (12) :4827-4843
[5]   Different stress responsive strategies to drought and heat in two durum wheat cultivars with contrasting water use efficiency [J].
Aprile, Alessio ;
Havlickova, Lenka ;
Panna, Riccardo ;
Mare, Caterina ;
Borrelli, Grazia M. ;
Marone, Daniela ;
Perrotta, Carla ;
Rampino, Patrizia ;
De Bellis, Luigi ;
Curn, Vladislav ;
Mastrangelo, Anna M. ;
Rizza, Fulvia ;
Cattivelli, Luigi .
BMC GENOMICS, 2013, 14
[6]   Protein degradation - an alternative respiratory substrate for stressed plants [J].
Araujo, Wagner L. ;
Tohge, Takayuki ;
Ishizaki, Kimitsune ;
Leaver, Christopher J. ;
Fernie, Alisdair R. .
TRENDS IN PLANT SCIENCE, 2011, 16 (09) :489-498
[7]   Roles of glycine betaine and proline in improving plant abiotic stress resistance [J].
Ashraf, M. ;
Foolad, M. R. .
ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2007, 59 (02) :206-216
[8]   DROUGHT TOLERANCE: ROLES OF ORGANIC OSMOLYTES, GROWTH REGULATORS, AND MINERAL NUTRIENTS [J].
Ashraf, M. ;
Akram, N. A. ;
Al-Qurainy, F. ;
Foolad, M. R. .
ADVANCES IN AGRONOMY, VOL 111, 2011, 111 :249-296
[9]   A RE-EXAMINATION OF RELATIVE TURGIDITY TECHNIQUE FOR ESTIMATING WATER DEFICITS IN LEAVES [J].
BARRS, HD ;
WEATHERLEY, PE .
AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES, 1962, 15 (03) :413-&
[10]   The role of amino acid metabolism during abiotic stress release [J].
Batista-Silva, Willian ;
Heinemann, Bjoern ;
Rugen, Nils ;
Nunes-Nesi, Adriano ;
Araujo, Wagner L. ;
Braun, Hans-Peter ;
Hildebrandt, Tatjana M. .
PLANT CELL AND ENVIRONMENT, 2019, 42 (05) :1630-1644