Role of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) in β-lactoglobulin aggregation

被引：2
|
作者：
Ma, Baoliang [1 ]
Wang, Xiaofei [1 ]
Liu, Yujie [1 ]
Li, Zhihong [1 ]
Zhang, Fan [1 ]
机构：
[1] Nanjing Agr Univ, Dept Phys, Coll Sci, Nanjing 210095, Jiangsu, Peoples R China
来源：
CHEMISTRY AND PHYSICS OF LIPIDS | 2018年 / 210卷
关键词：
Amyloid aggregation; Spherical aggregation; 1,2-dioleoy1-3-trimethylammonium-propane (DOTAP); beta-lactoglobulin (beta-LG); Critical micelle concentration (CMC); AMYLOID FIBRIL FORMATION; PROTEIN AGGREGATION; INDUCE; PH;
D O I：
10.1016/j.chemphyslip.2017.11.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Protein aggregation is a prevalent phenomenon. It is important to study protein aggregation under different solution conditions. In this study, using 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence spectra, we investigated the critical micelle concentration (CMC) of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). We also studied the effects of DOTAP on amyloid aggregation of beta-lactoglobulin using intrinsic fluorescence spectra, circular dichroism spectra, thioflavin T fluorescence, a Congo red binding assay and transmission electron microscopy. We observed that DOTAP had a dual role on beta-lactoglobulin amyloid aggregation. DOTAP inhibited the amyloid aggregation below the CMC, while it had the opposite effect above the CMC. Moreover, the results of transmission electron microscopy showed that spherical aggregates were formed above the CMC. These results led us to conclude that cationic lipids could be used as modulators of protein self-assembly.
引用
收藏
页码：76 / 81
页数：6
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