Pseudomonas urumqiensis sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia

A motile, Gram-stain-negative, fusiform-shaped bacterium, designated strain T3(T), was isolated from rhizosphere soil of Alhagi sparsifolia, collected from Xinjiang, PR China. Strain T3(T) grew at 15-42 degrees C, pH 4-9 and 1-6% (w/v) NaCl concentrations. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain T3(T) belonged to the genus Pseudomonas and showed highest similarity of 98.6 % to Pseudomonas azotifigens JCM 12708(T), followed by Pseudomonas balearica DSM 6083(T) (97.8 %), Pseudomonas matsuisoli JCM 30078(T) (97.7 %), Pseudomonas furukawaii KF707(T) (97.7 %), Pseudomonas tarimensis CCTCC AB 2013065(T) (97.3%) and Pseudomonas indica DSM 14015(T) (97.1 %). Analysis based on concatenated gene sequences of 16S rRNA, rpoB and gyrB further confirmed the phylogenetic assignment of strain T3(T). The Genome-to-Genome Distance Calculator results for P. azotifigens JCM12708(T) and P. balearica DSM 6083(T) were 28.7 +/- 4.4% and 24.1 +/- 2.4%, and the average nucleotide identity scores were 81.3 and 78.1 %. The major polar lipids of strain T3(T) were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant quinone was Q-9. The major fatty acids comprised summed feature 8 (C-18(:1)omega 6c/C-18:1 omega 7c; 37.7 %), summed feature 3 (C-16:1 omega 6c/C-16:1 omega 7c; 28.2 %), C-16:0 (15.6 %), C-12:0 (7.8 %), C(10:0)3-OH (3.0 %) and C(12:0)3-OH (2.6%). The G+C content of the genomic DNA of the type strain was 65.3 mol%. It is obvious from the phylogenetic, phenotypic and chemotaxonomic data that strain T3(T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas urumqiensis sp. nov., is proposed. The type strain is T3(T) (=ACCC 60124(T)=JCM 32830(T)).