Fabrication and Characterization of Biodegradable Nanofiber Containing Gelatin

被引：0

作者：

Yun, So Hee ^{[1
]}

Kim, Young-Jin ^{[1
,4
]}

Kwon, Oh Kyoung ^{[2
]}

Choi, Myung-Seok ^{[3
]}

Kim, Young-Jin ^{[1
,4
]}

Kwon, Oh Hyeong ^{[1
]}

机构：

[1] Kumoh Natl Inst Technol, Dept Polymer Sci & Engn, Gumi 730701, South Korea

[2] Gumi CHA Gen Hosp, Dept Surg, Gumi 730040, South Korea

[3] Konkuk Univ, Dept Chem & Mat Engn, Seoul 143701, South Korea

[4] Catholic Univ Daegu, Dept Biomed Engn, Hayang Eup 712702, Gyeongsan, South Korea

来源：

TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2008年 / 5卷 / 4-6期

关键词：

PHBV; gelatin; electrospinning; nanofiber scaffold;

D O I：

暂无

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Biodegradable polymeric nanofibrous matrices containing gelatin were fabricated by simple electrospinning method. The electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV)/gelatin (1: 1) nanofiber structure revealed randomly aligned fibers with average diameter of 600 nm determined and calculated by SEM and image analyzer. ATR-FTIR and ESCA mesurement were used to confirm the presence of gelatin in PHBV/gelatin nanofibers. Human fibroblasts' behavior on PHBV/gelatin nanofibrous matrices has been investigated. Fibroblasts were well attached on the surface of control PHBV and PHBV/gelatin nanofibers. Initial cell attachment on PHBV/gelatin nanofibers were significantly higher than that of control PHBV nanofibers, because gelatin has many RGD moiety to mediate cell attachment. Electrospinning of PHBV with gelatin significantly decreased water contact angle of control PHBV nanofiber. Cells could penetrate into the nanofibers and enabled 3D cell culture, due to the hydrophilic nature of PHBV/gelatin nanofibers. PHBV/gelatin nanofibers facilitated spacial distribution, and initial attachment of cells. From the above results, electrospinning of PHBV with gelatin is a promising method for application to cell culture matrices.

引用

页码：835 / 841

页数：7

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