Identification of an Extracellular Antifungal Protein from the Endophytic Fungus Colletotrichum sp DM06

被引:0
作者
Dey, Prabuddha [1 ]
Kamdar, Maulik R. [2 ]
Mandal, Santi M. [3 ]
Maiti, Mrinal K. [1 ,2 ]
机构
[1] Indian Inst Technol, Adv Lab Plant Genet Engn, Adv Technol Dev Ctr, Kharagpur 721302, W Bengal, India
[2] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India
[3] Indian Inst Technol, Cent Res Facil, Kharagpur 721302, W Bengal, India
关键词
Antifungal protein; Candida albicans; Colletotrichum sp DM-06; endophytic fungus; extracellular protein; pathogenic fungus; ANTIMICROBIAL PROTEIN; SECONDARY METABOLITES; CANDIDA-ALBICANS; PLANT; PEPTIDE; MICROORGANISMS; MECHANISMS; DEFENSINS; INSECT;
D O I
10.2174/092986613804725334
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An extracellular antifungal protein of 28 kDa (exAFP-C28) was identified from an endophytic fungus Colletotrichum sp. DM-06. After purification, the MIC value of exAFP-C28 against Candida albicans, a well-known human pathogenic fungus was found to be 32 mu g/mL that unaffected the human red blood cells. The antifungal activity associated with exAFP-C28 was manifested by the increased membrane permeability of C. albicans cells followed by disruption. Proteomics and bioinformatics analyses revealed that several peptide fragments of exAFP-C28 have identity with the bacterial 50S ribosomal protein L10, and a stretch of 55 amino acids of two peptide fragments corresponding to the N-terminus of L10 protein is capable of forming amphipathic helix required for membrane penetration. Taken together, our results suggest that the exAFP-C28 protein from Colletotrichum sp. DM-06 is a promising therapeutic agent in controlling candidiasis disease in animals including humans.
引用
收藏
页码:173 / 179
页数:7
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