Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry

被引：31

作者：

Hutson, J. R. ^{[2
]}

Aleksa, K.

Pragst, F. ^{[3
]}

Koren, G. ^{[1
,2
]}

机构：

[1] Hosp Sick Children, Div Clin Pharmacol Toxicol, Motherisk Lab, Toronto, ON M5C 1X8, Canada

[2] Univ Toronto, Inst Med Sci, Toronto, ON M5S 1A1, Canada

[3] Univ Hosp Charite, Inst Legal Med, Berlin, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 1-2期

关键词：

Fatty acid ethyl esters; Gas chromatography; Heads pace-solid-phase microextraction; Biomarker; Meconium; ETHANOL EXPOSURE; ALCOHOL-CONSUMPTION; HS-SPME; HAIR; PREVALENCE; BIOMARKERS; MARKERS; COCAINE; AGE;

D O I：

10.1016/j.jchromb.2008.10.056

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Meconium fatty acid ethyl esters (FAEEs) are currently used as biomarkers to detect heavy prenatal alcohol exposure. We introduce a novel technique to quantify FAEEs in meconium using heads pace-sol id-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This method improves on previous approaches by decreasing sample preparation time, eliminating the need for organic solvents, and reducing the required sample size. Using 50 mg of meconium, the detection limits of FAEEs ranged from 0.05 to 0.16 nmol/g and had good reproducibility making it ideal for routine analysis of clinical samples. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：8 / 12

页数：5

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