Dispensable, Redundant, Complementary, and Cooperative Roles of Dopamine, Octopamine, and Serotonin in Drosophila melanogaster

被引:44
作者
Chen, Audrey [2 ,3 ]
Ng, Fanny [4 ]
Lebestky, Tim [2 ]
Grygoruk, Anna [2 ]
Djapri, Christine [2 ]
Lawal, Hakeem O. [2 ]
Zaveri, Harshul A. [2 ]
Mehanzel, Filmon [2 ]
Najibi, Rod [2 ]
Seidman, Gabriel [2 ]
Murphy, Niall P. [2 ]
Kelly, Rachel L. [2 ]
Ackerson, Larry C. [2 ]
Maidment, Nigel T. [2 ]
Jackson, F. Rob [4 ]
Krantz, David E. [1 ,2 ]
机构
[1] Univ Calif Los Angeles, David Geffen Sch Med, Gonda Goldschmied Neurosci & Genet Res Ctr, Hatos Ctr Neuropharmacol,Dept Psychiat & Biobehav, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, David Geffen Sch Med, Semel Inst Neurosci & Human Behav, Hatos Ctr Neuropharmacol, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Dept Neurobiol, Los Angeles, CA 90095 USA
[4] Tufts Univ, Sch Med, Sackler Sch Grad Biomed Sci, Tufts Ctr Neurosci Res,Dept Neurosci, Boston, MA 02111 USA
基金
美国国家卫生研究院;
关键词
LOCOMOTOR-ACTIVITY; NEURAL CIRCUIT; MONOAMINE TRANSPORTERS; CELLULAR-DISTRIBUTION; PATTERN GENERATORS; CONTAINING NEURONS; GENE-EXPRESSION; BIOGENIC-AMINES; MOTOR-ACTIVITY; RECEPTOR OAMB;
D O I
10.1534/genetics.112.142042
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
To investigate the regulation of Drosophila melanogaster behavior by biogenic amines, we have exploited the broad requirement of the vesicular monoamine transporter (VMAT) for the vesicular storage and exocytotic release of all monoamine neurotransmitters. We used the Drosophila VMAT (dVMAT) null mutant to globally ablate exocytotic amine release and then restored DVMAT activity in either individual or multiple aminergic systems, using transgenic rescue techniques. We find that larval survival, larval locomotion, and female fertility rely predominantly on octopaminergic circuits with little apparent input from the vesicular release of serotonin or dopamine. In contrast, male courtship and fertility can be rescued by expressing DVMAT in octopaminergic or dopaminergic neurons, suggesting potentially redundant circuits. Rescue of major aspects of adult locomotion and startle behavior required octopamine, but a complementary role was observed for serotonin. Interestingly, adult circadian behavior could not be rescued by expression of DVMAT in a single subtype of aminergic neurons, but required at least two systems, suggesting the possibility of unexpected cooperative interactions. Further experiments using this model will help determine how multiple aminergic systems may contribute to the regulation of other behaviors. Our data also highlight potential differences between behaviors regulated by standard exocytotic release and those regulated by other mechanisms.
引用
收藏
页码:159 / 176
页数:18
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