Induction of apoptosis by D-limonene is mediated by inactivation of Akt in LS174T human colon cancer cells

被引：141

作者：

Jia, Shu-Sheng ^{[2
]}

Xi, Guang-Peng ^{[1
]}

Zhang, Ming ^{[2
]}

Chen, Yan-Bo ^{[2
]}

Lei, Bo ^{[2
]}

Dong, Xin-Shu ^{[3
]}

Yang, Yan-Mei ^{[1
]}

机构：

[1] Harbin Med Univ, Canc Res Inst, Harbin 150081, Heilongjiang, Peoples R China

[2] Harbin Med Univ, Affiliated Hosp 3, Dept Breast Surg, Harbin 150081, Heilongjiang, Peoples R China

[3] Harbin Med Univ, Affiliated Hosp 4, Harbin 150081, Heilongjiang, Peoples R China

来源：

ONCOLOGY REPORTS | 2013年 / 29卷 / 01期

关键词：

D-limonene; Akt; apoptosis; PHASE-I; INHIBITION; PATHWAY; CARCINOGENESIS; DEATH; GROWTH; MICE; HEPATOCARCINOGENESIS; PHOSPHORYLATION; CHEMOPREVENTION;

D O I：

10.3892/or.2012.2093

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

D-limonene is recognized as a potential chemotherapeutic agent, however, the details of this mechanism remain unclear. In this study, we investigated the effects of D-limonene on colon cancer cell viability and its potential mechanism of action in vitro. After 48 h of treatment, D-limonene suppressed the viability of LS174T cells in a dose-dependent manner and caused a dose-dependent apoptotic cell death. D-limonene activated caspase-3 and -9 and PARP cleavage in a dose-dependent manner. Moreover, an increase in Bax protein and cytosol cytochrome c from mitochondria and a decrease in Bcl-2 protein were observed following treatment with D-limonene. In addition, D-limonene decreased the levels of p-Akt (Ser473), p-Akt (Thr308) and p-GSK-3 beta (Ser9), suggesting that D-limonene induced apoptosis via the mitochondrial death pathway and the suppression of the PI3K/Akt pathway.

引用

页码：349 / 354

页数：6

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