Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles

被引：304

作者：

Wang, Zongxing ^{[1
,2
]}

Wu, Hung-jen ^{[1
]}

Fine, Daniel ^{[1
]}

Schmulen, Jeffrey ^{[1
]}

Hu, Ye ^{[1
]}

Godin, Biana ^{[1
]}

Zhang, John X. J. ^{[2
]}

Liu, Xuewu ^{[1
]}

机构：

[1] Methodist Hosp, Res Inst, Dept Nanomed, Houston, TX 77030 USA

[2] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA

来源：

LAB ON A CHIP | 2013年 / 13卷 / 15期

基金：

中国国家自然科学基金;

关键词：

EXOSOMES; CELL; BIOGENESIS; PROTEINS; CANCER; SERUM;

D O I：

10.1039/c3lc41343h

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We fabricated a microfluidic device consisting of ciliated micropillars, forming a porous silicon nanowire-on-micropillar structure. We demonstrated that the prototype device can preferentially trap exosome-like lipid vesicles, while simultaneously filtering out proteins and cell debris. Trapped lipid vesicles can be recovered intact by dissolving the porous nanowires in PBS buffer.

引用

页码：2879 / 2882

页数：4

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